In:
Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 116, No. suppl_16 ( 2007-10-16)
Abstract:
Objectives : The right ventricle (RV) fails quickly after increases in its afterload (pulmonary hypertension) compared to the LV (systemic hypertension). We hypothesized that the suboptimal performance of the hypertrophied RV (RVH) is caused by metabolic adaptation during hypertrophy. Mitochondria regulate metabolism, and mitochondrial membrane potential (ΔΨm) is a surrogate for mitochondrial function. Methods/Results : We measured ΔΨm in normal and hypertrophied myocardial specimens from 11 patients and in a rat model of RVH, using Tetramethylrhodamine methyl ester, a positively-charged dye that selectively localizes within the negatively charged mitochondria. ΔΨm was significantly higher in the normal LV compared to normal RV, and was the highest in RVH, both at the myocardium and isolated cardiomyocyte levels (p 〈 0.01 for both). The increase in ΔΨm was recapitulated in an in vitro model of hypertrophy, where isolated neonatal cardiomyocytes were exposed to phenylephrine. This increase in ΔΨm was reversed by both a direct inhibitor of NFAT (Nuclear-Factor-of-Activated-T-lymphocytes, a transcription factor that regulates cardiac metabolism in development and hypertrophy) and Dichloroacetate (DCA, a metabolic modulator that has been shown to depolarize mitochondria and inhibit NFAT, reversing the phenotype of disease in both cancer and pulmonary hypertension) (p 〈 0.01 for both). In ex-vivo Langendorff perfusions DCA acutely increased RV inotropy in rats with RVH (p 〈 0.01) but not normal RVs, suggesting that the increased ΔΨm in RVH might be associated with its suboptimal performance in patients with pulmonary hypertension. Conclusions : The significant differences in ΔΨm between RV, LV, and RVH, can be targeted metabolically by DCA to improve contractility in RVH and provide a framework for development of novel RV-specific therapies.
Type of Medium:
Online Resource
ISSN:
0009-7322
,
1524-4539
DOI:
10.1161/circ.116.suppl_16.II_193-d
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2007
detail.hit.zdb_id:
1466401-X
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