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1

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Proinflammatory cytokines increase the rate of glycolysis and adenosine-5′-triphosphate turnover in cultured rat enterocytes

Berg, Sören ; Sappington, Penny L. ; Guzik, Lynda J. ; [et al.]

Ovid Technologies (Wolters Kluwer Health) ; 2003

In: Critical Care Medicine Vol. 31, No. 4 ( 2003-04), p. 1203-1212

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In: Critical Care Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 31, No. 4 ( 2003-04), p. 1203-1212

Type of Medium: Online Resource

ISSN: 0090-3493

URL: Article

DOI: 10.1097/01.CCM.0000059647.92390.92

Language: English

Publisher: Ovid Technologies (Wolters Kluwer Health)

Publication Date: 2003

detail.hit.zdb_id: 2034247-0

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2

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Gallbladder as a novel source of stem cells of the hepatobiliary system

Manohar, Rohan Raoul ; Guzik, Lynda ; Stolz, Donna Beer ; [et al.]

Wiley ; 2011

In: The FASEB Journal Vol. 25, No. S1 ( 2011-04)

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In: The FASEB Journal, Wiley, Vol. 25, No. S1 ( 2011-04)

Type of Medium: Online Resource

ISSN: 0892-6638 , 1530-6860

URL: Issue

URL: Article

DOI: 10.1096/fsb2.v25.S1

DOI: 10.1096/fasebj.25.1_supplement.1002.3

Language: English

Publisher: Wiley

Publication Date: 2011

detail.hit.zdb_id: 1468876-1

SSG: 12

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3

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Duration and Magnitude of Hypotension and Monocyte Deactivation in Patients With Community-Acquired Pneumonia

Simon, Peter M. ; Delude, Russell L. ; Lee, MinJae ; [et al.]

Ovid Technologies (Wolters Kluwer Health) ; 2011

In: Shock Vol. 36, No. 6 ( 2011-12), p. 553-559

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In: Shock, Ovid Technologies (Wolters Kluwer Health), Vol. 36, No. 6 ( 2011-12), p. 553-559

Type of Medium: Online Resource

ISSN: 1073-2322

URL: Article

DOI: 10.1097/SHK.0b013e318235331e

Language: English

Publisher: Ovid Technologies (Wolters Kluwer Health)

Publication Date: 2011

detail.hit.zdb_id: 2011863-6

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4

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Polymerase η suppresses telomere defects induced by DNA damaging agents

Pope-Varsalona, Hannah ; Liu, Fu-Jun ; Guzik, Lynda ; [et al.]

Oxford University Press (OUP) ; 2014

In: Nucleic Acids Research Vol. 42, No. 21 ( 2014-12-01), p. 13096-13109

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In: Nucleic Acids Research, Oxford University Press (OUP), Vol. 42, No. 21 ( 2014-12-01), p. 13096-13109

Type of Medium: Online Resource

ISSN: 1362-4962 , 0305-1048

URL: Article

DOI: 10.1093/nar/gku1030

RVK:

WA 15000

Language: English

Publisher: Oxford University Press (OUP)

Publication Date: 2014

detail.hit.zdb_id: 1472175-2

SSG: 12

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5

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Human Bone Marrow-Derived Mesenchymal Stem Cells Display Enhanced Clonogenicity but Impaired Differentiation With Hypoxic Preconditioning

Boyette, Lisa B. ; Creasey, Olivia A. ; Guzik, Lynda ; [et al.]

Oxford University Press (OUP) ; 2014

In: Stem Cells Translational Medicine Vol. 3, No. 2 ( 2014-02-01), p. 241-254

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In: Stem Cells Translational Medicine, Oxford University Press (OUP), Vol. 3, No. 2 ( 2014-02-01), p. 241-254

Abstract: Stem cells are promising candidate cells for regenerative applications because they possess high proliferative capacity and the potential to differentiate into other cell types. Mesenchymal stem cells (MSCs) are easily sourced but do not retain their proliferative and multilineage differentiative capabilities after prolonged ex vivo propagation. We investigated the use of hypoxia as a preconditioning agent and in differentiating cultures to enhance MSC function. Culture in 5% ambient O2 consistently enhanced clonogenic potential of primary MSCs from all donors tested. We determined that enhanced clonogenicity was attributable to increased proliferation, increased vascular endothelial growth factor secretion, and increased matrix turnover. Hypoxia did not impact the incidence of cell death. Application of hypoxia to osteogenic cultures resulted in enhanced total mineral deposition, although this effect was detected only in MSCs preconditioned in normoxic conditions. Osteogenesis-associated genes were upregulated in hypoxia, and alkaline phosphatase activity was enhanced. Adipogenic differentiation was inhibited by exposure to hypoxia during differentiation. Chondrogenesis in three-dimensional pellet cultures was inhibited by preconditioning with hypoxia. However, in cultures expanded under normoxia, hypoxia applied during subsequent pellet culture enhanced chondrogenesis. Whereas hypoxic preconditioning appears to be an excellent way to expand a highly clonogenic progenitor pool, our findings suggest that it may blunt the differentiation potential of MSCs, compromising their utility for regenerative tissue engineering. Exposure to hypoxia during differentiation (post-normoxic expansion), however, appears to result in a greater quantity of functional osteoblasts and chondrocytes and ultimately a larger quantity of high-quality differentiated tissue.

Type of Medium: Online Resource

ISSN: 2157-6564 , 2157-6580

URL: Article

DOI: 10.5966/sctm.2013-0079

Language: English

Publisher: Oxford University Press (OUP)

Publication Date: 2014

detail.hit.zdb_id: 2642270-0

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6

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A Stochastic Model for Cancer Stem Cell Origin in Metastatic Colon Cancer

Odoux, Christine ; Fohrer, Helene ; Hoppo, Toshitaka ; [et al.]

American Association for Cancer Research (AACR) ; 2008

In: Cancer Research Vol. 68, No. 17 ( 2008-09-01), p. 6932-6941

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In: Cancer Research, American Association for Cancer Research (AACR), Vol. 68, No. 17 ( 2008-09-01), p. 6932-6941

Abstract: Human cancers have been found to include transformed stem cells that may drive cancer progression to metastasis. Here, we report that metastatic colon cancer contains clonally derived tumor cells with all of the critical properties expected of stem cells, including self-renewal and the ability to differentiate into mature colon cells. Additionally, when injected into mice, these cells initiated tumors that closely resemble human cancer. Karyotype analyses of parental and clonally derived tumor cells expressed many consistent (clonal) along with unique chromosomal aberrations, suggesting the presence of chromosomal instability in the cancer stem cells. Thus, this new model for cancer origin and metastatic progression includes features of both the hierarchical model for cancerous stem cells and the stochastic model, driven by the observation of chromosomal instability. [Cancer Res 2008;68(17):6932–41]

Type of Medium: Online Resource

ISSN: 0008-5472 , 1538-7445

URL: Article

DOI: 10.1158/0008-5472.CAN-07-5779

RVK:

XA 36000

RVK:

XA 10000

Language: English

Publisher: American Association for Cancer Research (AACR)

Publication Date: 2008

detail.hit.zdb_id: 2036785-5

detail.hit.zdb_id: 1432-1

detail.hit.zdb_id: 410466-3

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7

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Amnion-Derived Multipotent Progenitor Cells Inhibit Blood Monocyte Differentiation into Mature Dendritic Cells

Banas, Richard ; Miller, Carrie ; Guzik, Lynda ; [et al.]

SAGE Publications ; 2014

In: Cell Transplantation Vol. 23, No. 9 ( 2014-09), p. 1111-1125

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In: Cell Transplantation, SAGE Publications, Vol. 23, No. 9 ( 2014-09), p. 1111-1125

Abstract: Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit antigen-specific T-cell proliferation in a cell-to-cell contact-dependent fashion. Here we examine specific mechanisms involved in immunomodulation by AMP cells. We found that AMP cells significantly inhibited monocyte-derived myeloid dendritic cell (DC) maturation when placed in coculture. Cocultured monocytes retained the nondifferentiated macrophage marker CD14 while exhibiting significant reduction in DC maturation markers CD83 and CD1a, indicating an immature DC maturation state that is pivotal in determining its immune stimulatory or regulatory status. This effect was again dependent on cell-to-cell contact interaction. We also found a significant shift in cytokines present in the microenvironment of cocultures, which indicated a regulatory DC function rather than a stimulatory cell type. Here supernatants taken from AMP cell/monocyte cocultures yielded significant levels of regulatory cytokines, such as PGE 2 , IL-6, IL-10, and MIC-1. The soluble form of HLA-G was also found at higher levels in cocultures. In contrast, supernatants contained significantly less amounts of the T-cell-stimulating factor IL-12, which is normally produced by activated DCs. Interestingly, cocultured monocytes acquired significant expression of HLA-G on their cell surface over time. HLA-G has multifaceted immunological implications and may be a key molecule in influencing these cells to behave as regulatory DCs. Together, the influence of AMP cells on maturing DCs may favor a regulatory pathway that can be useful for therapeutic applications for immune-mediated disorders or transplantation therapies.

Type of Medium: Online Resource

ISSN: 0963-6897 , 1555-3892

URL: Article

DOI: 10.3727/096368913X670165

Language: English

Publisher: SAGE Publications

Publication Date: 2014

detail.hit.zdb_id: 2020466-8

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8

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Identification of a candidate stem cell in human gallbladder

Manohar, Rohan ; Li, Yaming ; Fohrer, Helene ; [et al.]

Elsevier BV ; 2015

In: Stem Cell Research Vol. 14, No. 3 ( 2015-05), p. 258-269

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In: Stem Cell Research, Elsevier BV, Vol. 14, No. 3 ( 2015-05), p. 258-269

Type of Medium: Online Resource

ISSN: 1873-5061

URL: Article

DOI: 10.1016/j.scr.2014.12.003

Language: English

Publisher: Elsevier BV

Publication Date: 2015

detail.hit.zdb_id: 2393143-7

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9

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Identification and expansion of a unique stem cell population from adult mouse gallbladder

Manohar, Rohan ; Komori, Junji ; Guzik, Lynda ; [et al.]

Ovid Technologies (Wolters Kluwer Health) ; 2011

In: Hepatology Vol. 54, No. 5 ( 2011-11), p. 1830-1841

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In: Hepatology, Ovid Technologies (Wolters Kluwer Health), Vol. 54, No. 5 ( 2011-11), p. 1830-1841

Abstract: The identification of resident stem cells in the mouse gallbladder is, to date, unexplored. In addition, the relationship between adult gallbladder stem cells and intrahepatic bile duct (IHBD) cells is not well understood. The aim of this study was to isolate stem cells from an adult mouse gallbladder and determine whether they were unique, compared to IHBD cells. By limiting dilution analyses and index sorts, we found that an EpCAM + CD49f hi epithelial cell subpopulation from primary gallbladder is enriched in colony-forming cells, compared to EpCAM + CD49f lo cells. EpCAM + CD49f hi cells expressed cluster of differentiation (CD)29, CD133, and stem cell antigen-1, but were negative for lineage markers CD31, CD45, and F4/80. Using a novel feeder cell-culture system, we observed long-term ( 〉 passage 20) and clonal expansion of the EpCAM + CD49f hi cells in vitro . In a matrigel differentiation assay, EpCAM + CD49f + cells expanding in vitro underwent organotypic morphogenesis forming ductular structures and cysts. These structures are similar to, and recapitulate a transport function of, primary gallbladder. EpCAM + CD49f + cells also engraft into the subcutaneous space of recipient mice. We compared primary gallbladder and IHBD cells by flow cytometry and found phenotypic differences in the expression of CD49f, CD49e, CD81, CD26, CD54, and CD166. In addition, oligonucleotide microarrays showed that the expanded EpCAM + CD49f + gallbladder cells and IHBD cells exhibit differences related to lipid and drug metabolism. Notable genes that were different are cytochrome P450, glutathione S -transferase, Indian hedgehog, and solute carrier family genes. Conclusion: We have isolated an epithelial cell population from primary mouse gallbladder with stem cell characteristics and found it to be unique, compared to IHBD cells. (Hepatology 2011)

Type of Medium: Online Resource

ISSN: 0270-9139

URL: Article

DOI: 10.1002/hep.24568

Language: English

Publisher: Ovid Technologies (Wolters Kluwer Health)

Publication Date: 2011

detail.hit.zdb_id: 1472120-X

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Berlin Brandenburg