In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 3 ( 2022-3-3), p. e0264533-
Abstract:
Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600–1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P 〈 0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm -1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0264533
DOI:
10.1371/journal.pone.0264533.g001
DOI:
10.1371/journal.pone.0264533.g002
DOI:
10.1371/journal.pone.0264533.g003
DOI:
10.1371/journal.pone.0264533.g004
DOI:
10.1371/journal.pone.0264533.g005
DOI:
10.1371/journal.pone.0264533.g006
DOI:
10.1371/journal.pone.0264533.g007
DOI:
10.1371/journal.pone.0264533.g008
DOI:
10.1371/journal.pone.0264533.t001
DOI:
10.1371/journal.pone.0264533.t002
DOI:
10.1371/journal.pone.0264533.s001
DOI:
10.1371/journal.pone.0264533.r001
DOI:
10.1371/journal.pone.0264533.r002
DOI:
10.1371/journal.pone.0264533.r003
DOI:
10.1371/journal.pone.0264533.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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