In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 15 ( 2024-4-18)
Abstract:
Cyanobacteria, which have a photoautotrophic lifestyle, are threatened by ultraviolet solar rays and the reactive oxygen species generated during photosynthesis. They can adapt to environmental conditions primarily because of their DNA damage response and repair mechanisms, notably an efficient homologous recombination repair system. However, research on double-strand break (DSB) repair pathways, including the Holliday junction (HJ) resolution process, in Synechocystis sp. PCC6803 is limited. Here, we report that SynRuvC from cyanobacteria Synechocystis sp. PCC6803 has classical HJ resolution activity. We investigated the structural specificity, sequence preference, and biochemical properties of SynRuvC. SynRuvC strongly preferred Mn 2+ as a cofactor, and its cleavage site predominantly resides within the 5′-TG↓(G/A)-3′ sequence. Interestingly, novel flap endonuclease and replication fork intermediate cleavage activities of SynRuvC were also determined, which distinguish it from other reported RuvCs. To explore the effect of SynRuvC on cell viability, we constructed a knockdown mutant and an overexpression strain of Synechocystis sp. PCC6803 ( synruvC KD and synruvC OE ) and assessed their survival under a variety of conditions. Knockdown of synruvC increased the sensitivity of cells to MMS, HU, and H 2 O 2 . The findings suggest that a novel RuvC family HJ resolvase SynRuvC is important in a variety of DNA repair processes and stress resistance in Synechocystis sp. PCC6803.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2024.1362880
DOI:
10.3389/fmicb.2024.1362880.s001
DOI:
10.3389/fmicb.2024.1362880.s002
DOI:
10.3389/fmicb.2024.1362880.s003
DOI:
10.3389/fmicb.2024.1362880.s004
DOI:
10.3389/fmicb.2024.1362880.s005
DOI:
10.3389/fmicb.2024.1362880.s006
DOI:
10.3389/fmicb.2024.1362880.s007
DOI:
10.3389/fmicb.2024.1362880.s008
DOI:
10.3389/fmicb.2024.1362880.s009
DOI:
10.3389/fmicb.2024.1362880.s010
DOI:
10.3389/fmicb.2024.1362880.s011
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2024
detail.hit.zdb_id:
2587354-4
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