In:
Developmental Immunology, Hindawi Limited, Vol. 6, No. 1-2 ( 1998), p. 25-39
Abstract:
Representing the most potent antigen-presenting cells, dendritic cells (DC) can now be generated from human blood monocytes. We recently presented a novel protocol employing GM-CSF, IL-4, and IFN- γ to differentiate monocyte-derived DC in vitro . Here, such cells are characterized in detail. Cells in culture exhibited both dendritic and veiled morphologies, the former being adherent and the latter suspended. Phenotypically, they were CD1a -/dim , CD11a + , CD11b ++ , CD11c + , CD14 dim/- , CD16 a-/dim , CD18 + , CD32 dim/- , CD33 + , CD40 + , CD45R0 + , CD50 + , CD54 + , CD64 -/dim , CD68 + , CD71 + , CD80 dim , CD86 +/++ , MHC class I ++/+++ HLA-DR ++/+++ HLA-DP + , and HLA-DQ + . The DC stimulated a strong allogeneic T-cell response, and further evidence for their autologous antigen-specific stimulation is discussed. Although resembling a mature CD 11c + CD45R0 + blood DC subset identified earlier, their differentiation in the presence of the Thl and Th2 cytokines IFN- γ and IL-4 indicates that these DC may conform to mature mucosal DC.
Type of Medium:
Online Resource
ISSN:
1044-6672
Language:
English
Publisher:
Hindawi Limited
Publication Date:
1998
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