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  • 1
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    Online Resource
    Seasonal assessment of selected trace elements in grass carp (Ctenopharyngodon idella) blood and their effects on the biochemistry and oxidative stress markers
    Springer Science and Business Media LLC ; 2023
    In:  Environmental Monitoring and Assessment Vol. 195, No. 12 ( 2023-12)
    Details
    In: Environmental Monitoring and Assessment, Springer Science and Business Media LLC, Vol. 195, No. 12 ( 2023-12)
    Abstract: Environmental pollution by anthropogenic activity is still a highly relevant global problem. Aquatic animals are a specifically endangered group of organisms due to their continuous direct contact with the contaminated environment. Concentrations of selected trace elements in the grass carp ( Ctenopharyngodon idella ) ( n = 36) blood serum/clot were monitored. Possible effects of the elements on selected biochemical and oxidative markers were evaluated. The concentrations of trace elements (Al, Ba, Be, Bi, Cd, Co, Cr, Cu, Fe, Ga, Mn, Mo, Ni, Pb, Sr, Tl, and Zn) were analysed in the fish blood serum and blood clot by inductively coupled plasma optical emission spectrometry (ICP OES). A general scheme of decreasing concentrations of trace elements in the blood serum samples was: Zn ˃ Fe ˃ Sr ˃ Ba ˃ Ni ˃ Al ˃ Cu ˃ Be ˃ Co; 〈 LOQ (below limit of quantification): Bi, Cd, Cr, Ga, Mn, Mo, Pb, Tl; and in the case of the blood clot, the scheme was as follows: Fe ˃ Zn ˃ Sr ˃ Al ˃ Ni ˃ Ba ˃ Cu ˃ Be ˃ Co ˃ Mn; 〈 LOQ (below limit of quantification): Bi, Cd, Cr, Ga, Mo, Pb, Tl. Significant differences among the seasons were detected. The Spearman R correlation coefficients and linear or non-linear regression were used to evaluate direct relationships between trace elements and selected blood biomarkers. The correlation analysis between biochemical parameters (Na, K, P, Mg, AST, ALT, ALP, GGT, TAG, TP, urea, glucose) and trace elements (Al, Ba, Be, Cu, Fe, Ni, Sr, and Zn) concentrations confirmed statistically significant interactions in both seasons (summer and autumn). The regression analysis between oxidative stress markers (ROS, GPx, creatinine, uric acid, and bilirubin) and elements (Al, Ba, Co, Cu, Fe, Ni, and Sr) content confirmed statistically significant interactions. The results point to numerous connections between the observed elements and the physiological parameters of freshwater fish.
    Type of Medium: Online Resource
    ISSN: 0167-6369 , 1573-2959
    URL: Article
    DOI: 10.1007/s10661-023-12152-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2012242-1
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  • 2
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    Online Resource
    Antifungal activity of essential oils against selected terverticillate penicillia
    Institute of Rural Health ; 2015
    In:  Annals of Agricultural and Environmental Medicine Vol. 22, No. 1 ( 2015-2-24), p. 38-42
    Details
    In: Annals of Agricultural and Environmental Medicine, Institute of Rural Health, Vol. 22, No. 1 ( 2015-2-24), p. 38-42
    Type of Medium: Online Resource
    ISSN: 1232-1966 , 1898-2263
    URL: Article
    DOI: 10.5604/12321966.1141367
    Language: Unknown
    Publisher: Institute of Rural Health
    Publication Date: 2015
    detail.hit.zdb_id: 2109674-0
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  • 3
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    Online Resource
    BARLEY VARIETIES DISCRIMINATION USING MALDI-TOF MASS SPECTROMETRY
    Slovak University of Agriculture in Nitra ; 2019
    In:  Journal of microbiology, biotechnology and food sciences Vol. 8, No. 6 ( 2019-06-01), p. 1347-1351
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 8, No. 6 ( 2019-06-01), p. 1347-1351
    Abstract: Barley is a widely used foodstuff and it is the most important component in malting and brewing. Purity of barley grains in malting and brewing is important in relation to technological processing. Therefore, an effective analysis of barley purity has high priority. The aim of this study was the discrimination of barley varieties using Matrix Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS). Eight barley varieties, Kangoo, Laudis, Malz, Marthe, Odyssey, Overture, Sebastian and Wintmalt were investigated. Several protein extraction methods, barley samples preparation for MALDI-TOF MS, reproducibility, influence of time on the hordein quality spectrum and groups of hordeins mass spectrum suitable for barley discrimination were tested. The results showed that removal of albumins and globulins does not affect the resulting hordein spectrum, but time decreases the hordein spectrum intensity. Preparation of barley samples has the highest priority. The most important factor is the selection of the right matrix and its use. B hordeins should be used for the discrimination of Barley varieties. Local Barley database based on B hordeins can be created but the protein spectra must be obtained with the highest purity and the highest reproducibility. The right set up of MALDI Biotyper software can lead to local Barley database establishment. Also, Barley’s B hordeins were used for the creation of a relatedness dendrogram. These obtained results showed that MALDI-TOF MS is a very usefull and powefull method for Barley varieties discrimination with a high reproducibility.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2019.8.6.1347-1351
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2019
    detail.hit.zdb_id: 2641525-2
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  • 4
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    Online Resource
    PROXIMATE, MINERAL AND FUNCTIONAL PROPERTIES OF TIGER NUT FLOUR EXTRACTED FROM DIFFERENT TIGER NUTS CULTIVARS
    Slovak University of Agriculture in Nitra ; 2019
    In:  Journal of microbiology, biotechnology and food sciences Vol. 9, No. 3 ( 2019-12-01), p. 653-656
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 9, No. 3 ( 2019-12-01), p. 653-656
    Abstract: Tiger nut flour extracted from (black, brown and yellow) cultivars using attrition milling process was investigated. Tiger nut flours labeled Ab, Bb and Cy for black, brown and yellow cultivars were produced respectively. Proximate composition, mineral elements contents, functional properties of the tiger nut flours were determined. There was significant (p
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2019/20.9.3.653-656
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2019
    detail.hit.zdb_id: 2641525-2
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  • 5
    Online Resource
    Online Resource
    TOPINAMBUR (THE JERUSALEM ARTICHOKE): NUTRITIONAL VALUE AND ITS APPLICATION IN FOOD PRODUCTS: AN UPDATED TREATISE
    Slovak University of Agriculture in Nitra ; 2021
    In:  Journal of microbiology, biotechnology and food sciences Vol. 10, No. 6 ( 2021-06-01), p. e4737-
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 10, No. 6 ( 2021-06-01), p. e4737-
    Abstract: This article presents a review of the scientific literature on the nutritional value of topinambur (Jerusalem artichoke) and its potential use in the formulation of food products. The scientific review covers the chemical, amino-acid, vitamin, mineral composition of topinambur, its pharmacological and therapeutic and prophylactic effect on the human organism. The distinctive features of topinambur are the content of more than 20% of dry substances, among which up to 80% contains a polymeric homologue of fructose - inulin. The content of pectin substances varies up to 11% of dry weight. Consumption of topinambur as a part of foodstuffs has a beneficial effect on absolutely all the chains and causes of diseases of the digestive system, cardiovascular system and liver. A number of findings about the use of topinambur roots in the process of preparation of meat, milk, confectionary and bread products are presented, its positive effect on the nutritional value and consumer properties of the final product is characterized. Utilization of Jerusalem artichoke in formulation of various food products is a good way to increase the alternative usage area of them.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.4737
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2021
    detail.hit.zdb_id: 2641525-2
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  • 6
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    Online Resource
    ANALYSIS OF REPORTED FOOD FRAUD IN THE GLOBAL BEEF SUPPLY CHAIN 2000-2020
    Slovak University of Agriculture in Nitra ; 2022
    In:  Journal of microbiology, biotechnology and food sciences ( 2022-02-22), p. e5872-
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, ( 2022-02-22), p. e5872-
    Abstract: Relevant food notifications published under the Rapid Alert System for Food and Feed (RASFF) for 2000-2020 were selected and analyzed to determine their overall formula. Fraud/counterfeiting was categorized according to the type of fraud, its location in the food production chain, the reporting country, and the origin of the food. "Adulteration" was the most common type of fraud in the beef industry; it accounted for 70% of all documented reports. According to the classification of supply chain messages, 59% of all cases were attributed to secondary processing, of which 83% were cases of "adulteration". The most common counterfeiting practices included products manufactured/packaged in non-approved areas, without appropriate control or documentation, products with fraudulent health certificates, and substitution of meat types or the addition of veterinary medicines.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.55251/jmbfs.5872
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2022
    detail.hit.zdb_id: 2641525-2
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  • 7
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    Online Resource
    THE IN VITRO ANTIFUNGAL ACTIVITY OF LACTOBACILLUS SPP. AGAINST ASPERGILLUS OCHRACEUS GROWTH AND ITS OCHRATOXIN A PRODUCTION
    Slovak University of Agriculture in Nitra ; 2022
    In:  Journal of microbiology, biotechnology and food sciences ( 2022-09-16), p. e9460-
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, ( 2022-09-16), p. e9460-
    Abstract: This study was aimed to monitor the inhibitory effect of selected species of the genus Lactobacillus on growth and ochratoxin A production of the microscopic filamentous fungus Aspergillus ochraceus (SLO-B-245). Nine species of the genus Lactobacillus were used in this study, namely L. bifermentatus. L. perolens, L. reuteri, L. paracasei, L. paraplantarum, L. plantarum, L. salivarius, L. fructivorans and L. pentosus. All strains were isolated from dairy products and their identification was performed by MALDI TOF MS (Bruker Daltonics, Germany, Maldi Biotyper). The antifungal activity was carried out using the overlay technique. Solid culture media inoculated with bacterial and fungal inocula were cultivated under aerobic conditions at 37 ºC for 8 days. Diameters (Ø mm) of the grown fungal colonies were measured every two days. To determine the inhibition of ochratoxin A production, the thin-layer chromatography method (TLC) was used. L. plantarum and L. paraplantarum were able to inhibit the growth of A. ochraceus (SLO-B-245) completely (100% inhibition), while L. fructivorans appeared to be the least effective (10.73%). Interestingly, the latter one was able to completely inhibit or partially suppress ochratoxin A production. We hope that our results can contribute to the search for harmless substances for the biological control of fungi in food. Lactobacilli and similar organisms could be very promising alternatives for food preservation.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.55251/jmbfs.9460
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2022
    detail.hit.zdb_id: 2641525-2
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  • 8
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    Online Resource
    ASSESSMENT OF THE PHYTOCHEMICAL AND FUNCTIONAL PROPERTIES OF PRE-TREATED ADUWA (BALANITES AEQYPTIACA) SEED MEAL FOUR
    Slovak University of Agriculture in Nitra ; 2019
    In:  Journal of microbiology, biotechnology and food sciences Vol. 9, No. 2 ( 2019-10-01), p. 354-358
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 9, No. 2 ( 2019-10-01), p. 354-358
    Abstract: Effects of pretreatments on the phytochemical properties of Balanites aeqyptiaca seed meal were investigated. Balanites aegyptiaca, belongs to the family Balaniteceae, that grows in tropical zones and is employed as animal forage. Its leaves are cooked and the seeds contains high quality oil and protein content. In this experiment, Balanites aeqyptiaca seeds were subjected to three treatments: First was toasting just before milling, oil extraction and finally meal cake production. Second was boiling, drying, toasting, milling, oil extracting and finally meal cake production. The third one was soaking overnight at ambient temperature, toasting, oil extraction and ultimately production of meal cake. In addition, raw seeds were marked as control. The main constituents were reported as 13.3-24.01% for water, 14.4-24% for lipid, 1.43-4.02% for crude fiber, 7.36-8.97% for moisture content and 43.79-52.50 % for carbohydrates. Further experiments also were performed to evaluate photo-chemicals like saponnins, alkaloids, flavonoids, phenols and tannins in pre-treated samples. These compounds were ranged between 5.81-8.63 mg/g in saponnins, 0.65-1.42 mg/g in alkaloids, 9.28-11.17 mg/g in flavonoids, 2.00-4.00 mg/g in phenols and 0.00-2.40 mg/g in tannins. To characterize deeply the pre-treated samples, some of the functional properties like bulk density, least gelation capacity, oil and water absorption were also examined. The results indicated that foaming capacity ranged between 16.06-67.3 %, bulk density value ranged from 60-75 g/ml, Least gelation capacity was 11.03-12.74 g/ml, oil absorption and water absorption capacity values ranged between 0.00-0.01g/ml and 0.08-0.26g/ml respectively.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.2019.9.2.354-358
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2019
    detail.hit.zdb_id: 2641525-2
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  • 9
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    Online Resource
    POTENTIAL EFFECT OF THYME AND OREGANO ESSENTIAL OILS TO ANTIMICROBIAL ACTIVITY AND YEAST ENZYMATIC ANTIOXIDATIVE SYSTEM
    Slovak University of Agriculture in Nitra ; 2021
    In:  Journal of microbiology, biotechnology and food sciences Vol. 11, No. 3 ( 2021-12-01), p. e5583-
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, Vol. 11, No. 3 ( 2021-12-01), p. e5583-
    Abstract: This in vitro study was aimed to assess the effects of Thymus vulgaris L. and Origanum vulgare L. essential oils (EOs) on cell viability and enzymatic antioxidative system in yeast Saccharomyces cerevisiae. Yeasts were cultivated with different concentrations of thyme (Thymus vulgaris L.) (0.05 – 1.00 µl/ml) and oregano (Origanum vulgare L.) (0.05 – 0.70 µl/ml) EOs for 24 hours. For the present study, gas chromatography-mass spectrometry (GC-MS) analysis was used to quantify chemical composition of EOs. The main components of thyme essential oil were caryophyllene (7.94%), p-cymene (18.55%), and thymol (46.55%). In essential oil of oregano, there was found p-cymene (8.43%) and carvacrol (69.99%) mainly. The antimicrobial activity of selected EOs was investigated by the microbroth dilution method using 96 well microtiter plates. The most potent essential oil able to inhibit growth of Saccharomyces cerevisiae was oregano EO with the values of MIC50 0.468845 µl/ml and MIC90 0.501845 µl/ml. Also, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were assessed. According to our results, Thymus vulgaris L. significantly increased the SOD levels at 0.2, 0.4, 0.5 to 0.9 µl/ml and GPx production at 0.05 to 0.7 µl/ml (P 〈 0.001). In case of Origanum vulgare L., significant increase of SOD production at 0.05 - 0.2 µl/ml (P 〈 0.001) was observed. This essential oil also elevated GPx concentration in all experimental groups (P 〈 0.001) significantly. Data from this study suggest that selected EOs have significant effects on enzymatic antioxidants that may affect the first-line cell defense system against oxidative stress.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.15414/jmbfs.5583
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2021
    detail.hit.zdb_id: 2641525-2
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  • 10
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    Online Resource
    VITALITY AND BACTERIOLOGICAL PROFILE OF LIPTOV BOLD-SPOTTED RABBIT SEMEN
    Slovak University of Agriculture in Nitra ; 2022
    In:  Journal of microbiology, biotechnology and food sciences ( 2022-12-21), p. e9724-
    Details
    In: Journal of microbiology, biotechnology and food sciences, Slovak University of Agriculture in Nitra, ( 2022-12-21), p. e9724-
    Abstract: Since semen quality is important for the successful artificial insemination (AI) or cryopreservation, we focused on the evaluation of Slovak national rabbit breeds, New Zealand rabbit and Liptov Bold-Spotted rabbit semen as a possible source of gene reserve. Besides standard sperm traits such as motility, viability, acrosome integrity, and oxidative damage (ROS), bacterial profiles were also assessed in semen samples of New Zealand rabbit (control; n=2; N1 and N2) and Liptov Bold-Spotted rabbit (experimental; n=2; L2 and L4). After initial analysis, samples were divided into good ( 〉 70%) and poor ( 〈 70%) quality samples according to motility and viability. The results showed significantly higher (P 〈 0.05) totally motile spermatozoa in N1, N2 (76.53±1.22%; 77.30±1.22%) compared to L2 (35.86±5.39%) as well as higher progressively motile spermatozoa in N1 (60.53±1.35%) and N2 (68.13±4.14%) compared to L2 (24.16±1.95%). However, no significant differences in L4 (45.11±4.05%) compared to other groups were recorded. The proportion of dead/live, apoptotic, acrosome damaged, mitochondrial active and ROS produced rabbit sperm was assessed via flow cytometry using fluorescent dyes: DRAQ7, SYBR-14, Yo-Pro-1, PNA, MitoTracker and CellROX, respectively. Significant (p 〈 0.05) decrease of live sperm was observed in L4 (42.73±6.89%) compared to N1 (75.22±5.75%) sample. In adittion, significant (p 〈 0.05) increased presence of dead sperm was in L4 (41.20±10.16%) group compared to N2 (11.16±3.01%). The activity of rabbit sperm mitochondria assessed via mitochondrial membrane potential was significantly (p 〈 0.05) higher in N1 (93.37±1.46%) and N2 (94.35±0.74%) compared to L4 (57.34±1.92). The results from the bacteriological analysis showed that the sperm in good quality (N1 and N2) contained mainly microbial genus such as Brevundimonas, Brevibacterium, Bacillus or Achromobacter. Other samples with poor quality (L2, L4) contained different kind of bacterial genus such as Pseudomonas, Delftia, Acidovorax, Acinetobacter or Staphylococcus. Although the microorganism profile appeared different, obligate pathogenic species were not present. Therefore, further analyses are needed to find out the reason of poor semen quality.
    Type of Medium: Online Resource
    ISSN: 1338-5178
    URL: Article
    DOI: 10.55251/jmbfs.9724
    Language: Unknown
    Publisher: Slovak University of Agriculture in Nitra
    Publication Date: 2022
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