In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 10 ( 2021-10-20), p. e0258812-
Abstract:
Secretory IgA (SIgA) is released into mucosal surfaces where its function extends beyond that of host defense to include the shaping of resident microbial communities by mediating exclusion/inclusion of respective microbes and regulating bacterial gene expression. In this capacity, SIgA acts as the fulcrum on which host immunity and the health of the microbiota are balanced. We recently completed an analysis of the gut and salivary IgA-Biomes (16S rDNA sequencing of SIgA-coated/uncoated bacteria) in Mexican-American adults that identified IgA-Biome differences across the glycemic spectrum. As Th17:Treg ratio imbalances are associated with gut microbiome dysbiosis and chronic inflammatory conditions such as type 2 diabetes, the present study extends our prior work by examining the impact of Th17:Treg ratios (pro-inflammatory:anti-inflammatory T-cell ratios) and the SIgA response (Th17:Treg-SIgA axis) in shaping microbial communities. Examining the impact of Th17:Treg ratios (determined by epigenetic qPCR lymphocyte subset quantification) on the IgA-Biome across diabetes phenotypes identified a proportional relationship between Th17:Treg ratios and alpha diversity in the stool IgA-Biome of those with dysglycemia, significant changes in community composition of the stool and salivary microbiomes across glycemic profiles, and genera preferentially abundant by T-cell inflammatory phenotype. This is the first study to associate epigenetically quantified Th17:Treg ratios with both the larger and SIgA-fractionated microbiome, assess these associations in the context of a chronic inflammatory disease, and offers a novel frame through which to evaluate mucosal microbiomes in the context of host responses and inflammation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0258812
DOI:
10.1371/journal.pone.0258812.g001
DOI:
10.1371/journal.pone.0258812.g002
DOI:
10.1371/journal.pone.0258812.g003
DOI:
10.1371/journal.pone.0258812.g004
DOI:
10.1371/journal.pone.0258812.g005
DOI:
10.1371/journal.pone.0258812.g006
DOI:
10.1371/journal.pone.0258812.g007
DOI:
10.1371/journal.pone.0258812.g008
DOI:
10.1371/journal.pone.0258812.g009
DOI:
10.1371/journal.pone.0258812.s001
DOI:
10.1371/journal.pone.0258812.s002
DOI:
10.1371/journal.pone.0258812.s003
DOI:
10.1371/journal.pone.0258812.s004
DOI:
10.1371/journal.pone.0258812.s005
DOI:
10.1371/journal.pone.0258812.s006
DOI:
10.1371/journal.pone.0258812.s007
DOI:
10.1371/journal.pone.0258812.r001
DOI:
10.1371/journal.pone.0258812.r002
DOI:
10.1371/journal.pone.0258812.r003
DOI:
10.1371/journal.pone.0258812.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Bookmarklink