In:
HortScience, American Society for Horticultural Science, Vol. 35, No. 4 ( 2000-07), p. 745-748
Abstract:
Callus formation and adventitious shoot regeneration in vitro from mature stored seed were evaluated in eight ornamental cherry ( Prunus ) taxa: P. campanulata Maxim . , P. maackii Rupr., P. sargentii Rehd., P. serrula Franch., P. serrulata Lindl., P. subhirtella Miq., P. virginiana L. , and P. yedoensis Matsum . Several portions of the embryo (cotyledons and hypocotyl sections) and nine combinations of growth regulators (BA, 2,4-D, IBA, NAA, and TDZ) were compared. Effects of embryo portions and growth regulator treatments were generally small within taxa, but shoot formation differed among taxa. About 20% to 50% of the embryos from P. virginiana and P. serrula and ≈5% to 30% of those from P. maackii produced shoots. The other taxa generally did not produce shoots. Regeneration from mature stored seed in the responsive taxa represents a potential system for genetic transformation. Chemical names used: 6-benzyladenine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA); thidiazuron (TDZ).
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.35.4.745
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2000
detail.hit.zdb_id:
2040198-X
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