In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 105, No. 41 ( 2008-10-14), p. 15890-15895
Abstract:
Interferon regulatory factor (IRF) 4 is a member of the IRF family of transcription factors and plays critical roles in the development of CD4 + T cells into Th2 and Th17 cells. Using the infection model of Nippostrongyrus brasiliensis , we have confirmed the critical roles of IRF-4 in Th2 development in vivo by using IRF-4 −/− BALB/c mice. However, naïve IRF-4 −/− CD4 + T cells produced Th2 cytokines, including IL-4, IL-5, and IL-10, but not IL-2 or IFN-γ, at levels higher than wild-type BALB/c CD4 + T cells in response to T cell receptor stimulation. In contrast, effector/memory IRF-4 −/− CD4 + T cells did not exhibit increased production of Th2 cytokines. Knockdown of IRF-4 expression by using small interfering RNA promoted IL-4 production in naïve CD4 + T cells but inhibited it in effector/memory CD4 + T cells. These results indicate that IRF-4 plays differential roles in the regulation of Th2 cytokine production in naïve CD4 + T cells and effector/memory CD4 + T cells. IRF-4 inhibits Th2 cytokine production in naïve CD4 + T cells, whereas it promotes Th2 cytokine production in effector/memory CD4 + T cells.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.0803171105
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2008
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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