In:
Respiration, S. Karger AG, Vol. 71, No. 4 ( 2004), p. 360-366
Abstract:
〈 i 〉 Background: 〈 /i 〉 Patients with cystic fibrosis (CF) are at an increased risk of pulmonary colonisation by opportunistic micro-organisms. Using specialised methods, the black yeast 〈 i 〉 Exophiala dermatitidis 〈 /i 〉 could consistently be cultured from CF patients. Isolation rates from sputum samples ranged between 1.8 and 15.7%. Occasionally, infection could be recognised. 〈 i 〉 Objectives: 〈 /i 〉 This study aimed at investigating the isolation rates of 〈 i 〉 E. dermatitidis 〈 /i 〉 in samples taken from CF patients at the University of Bonn, Germany. 〈 i 〉 Methods: 〈 /i 〉 Altogether, 439 respiratory specimens taken from 81 CF patients were screened for the occurrence of 〈 i 〉 E. dermatitidis 〈 /i 〉 over a period of 18 months. For the selective isolation of this fungus erythritol-chloramphenicol agar (ECA) produced in house was applied. 〈 i 〉 Results: 〈 /i 〉 The isolation rate of 〈 i 〉 E. dermatitidis 〈 /i 〉 was 1.1% from all specimens, 1.6% from all sputum samples and 6.2% in all patients examined. 〈 i 〉 Conclusions: 〈 /i 〉 Prior to the introduction of ECA, 〈 i 〉 E. dermatitidis 〈 /i 〉 had never been isolated in our laboratory, either from CF, or from any other patient. During this study, 〈 i 〉 E. dermatitidis 〈 /i 〉 was found to colonise the respiratory tract of some CF patients. The use of additional selective culture media is necessary for the recognition of uncommon fungi, e.g. 〈 i 〉 E. dermatitidis 〈 /i 〉 , in CF patients.
Type of Medium:
Online Resource
ISSN:
0025-7931
,
1423-0356
Language:
English
Publisher:
S. Karger AG
Publication Date:
2004
detail.hit.zdb_id:
1464419-8
detail.hit.zdb_id:
206674-9
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