In:
Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 11 ( 2021-12-9)
Abstract:
In previous experiments, we identified the effect of deletion of the Zbtb1 gene on circRNAs and microRNAs. In this study, we examined the expression profiles of lncRNAs and mRNAs using the RNA-seq method for Zbtb1-deficient EL4 cells and performed a clustering analysis of differentially expressed lncRNAs and mRNAs. GO term histograms and KEGG scatter plots were drawn. For the experimental results, a joint analysis was performed, which predicted the regulatory relationships among lncRNAs, mRNAs, microRNAs and circRNAs. For the regulatory relationship between lncRNAs and target genes, the chromatin structure and the degree of openness were verified for the possible target gene locations regulated by lncRNA using experimental methods such as Hi-C and ATAC-seq. Ultimately, the possible differential regulation of the Brcal and Dennd5d genes by lncRNAs and the differential changes in transcription factor binding sites in the promoter region were identified. For neRNA-regulated target genes with significantly differentially expressed mRNAs, a combined screen was performed, and the final obtained candidate target genes were subjected to GO and KEGG term enrichment analyses. Our results illustrate that the Zbtb1 gene can not only function as a regulatory factor but also regulate EL4 cells from multiple perspectives based on ceRNA theory.
Type of Medium:
Online Resource
ISSN:
2235-2988
DOI:
10.3389/fcimb.2021.806290
DOI:
10.3389/fcimb.2021.806290.s001
DOI:
10.3389/fcimb.2021.806290.s002
DOI:
10.3389/fcimb.2021.806290.s003
DOI:
10.3389/fcimb.2021.806290.s004
DOI:
10.3389/fcimb.2021.806290.s005
DOI:
10.3389/fcimb.2021.806290.s006
DOI:
10.3389/fcimb.2021.806290.s007
DOI:
10.3389/fcimb.2021.806290.s008
DOI:
10.3389/fcimb.2021.806290.s009
DOI:
10.3389/fcimb.2021.806290.s010
DOI:
10.3389/fcimb.2021.806290.s011
DOI:
10.3389/fcimb.2021.806290.s012
DOI:
10.3389/fcimb.2021.806290.s013
DOI:
10.3389/fcimb.2021.806290.s014
DOI:
10.3389/fcimb.2021.806290.s015
DOI:
10.3389/fcimb.2021.806290.s016
DOI:
10.3389/fcimb.2021.806290.s017
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2619676-1
Bookmarklink