In:
Asia-Pacific Journal of Clinical Oncology, Wiley, Vol. 16, No. 2 ( 2020-04)
Abstract:
Immune suppression based on alternative regulation of immune checkpoint proteins, for example, programmed cell death receptor‐1 (PD‐1) and cytotoxic T lymphocyte–associated molecule‐4 (CTLA‐4), which results in T‐cell exhaustion, contributes to cancer development and progression. In this study, we sought to characterize the distribution of CTLA‐4 and T‐cell lymphocyte activation gene‐3 (LAG‐3) expression on exhausted T cells in different T‐cell subsets from patients with acute myeloid leukemia (AML). Methods The coexpression of CTLA‐4 and LAG‐3 on exhausted CD244 + and CD57 + T cells from the CD3 + , CD4 + , and CD8 + T‐cell subsets in peripheral blood from 12 patients with newly diagnosed AML was analyzed by multicolor flow cytometry assay. Results A significantly higher percentage of CTLA‐4 + CD3 + , CD4 + and CD8 + T cells was found in patients with AML. In addition, higher numbers of both CTLA‐4 + CD244+ and CTLA‐4 + CD57 + CD3 + T cells were detected. Interestingly, the increased CTLA‐4 + CD244 + T cells were predominantly CD4 + T cells. In contrast, the increased CTLA‐4 + CD57 + T cells primarily consisted of the CD8 + T‐cell subset. A high proportion of LAG‐3 + T cells was found in only a few cases with AML; however, a significantly higher proportion of coexpression of CTLA‐4 and LAG‐3 in the CD3 + and CD8 + T‐cell subsets was detected. Conclusion We for the first time observed higher CTLA‐4 + CD244 + CD4 + , CTLA‐4 + CD57 + CD8 + , CTLA‐4 + LAG‐3 + CD3 + and CTLA‐4 + LAG‐3 + CD8 + T cells in patients with AML, whereas the upregulated expression of LAG‐3 on T cells was only found in a subset of the cases. These data may provide further information by complementing the heterogeneity of immune checkpoints expression in AML.
Type of Medium:
Online Resource
ISSN:
1743-7555
,
1743-7563
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
2187409-8
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