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Apical constriction is necessary for crypt formation in small intestinal organoids

Hartl, Leonie ; Huelsz-Prince, Guizela ; van Zon, Jeroen ; [et al.]

Elsevier BV ; 2019

In: Developmental Biology Vol. 450, No. 2 ( 2019-06), p. 76-81

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In: Developmental Biology, Elsevier BV, Vol. 450, No. 2 ( 2019-06), p. 76-81

Type of Medium: Online Resource

ISSN: 0012-1606

URL: Article

DOI: 10.1016/j.ydbio.2019.03.009

Language: English

Publisher: Elsevier BV

Publication Date: 2019

detail.hit.zdb_id: 1463203-2

SSG: 12

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Cells change their sensitivity to an EGF morphogen gradient to control EGF-induced gene expression

van Zon, Jeroen Sebastiaan ; Kienle, Simone ; Huelsz-Prince, Guizela ; [et al.]

Springer Science and Business Media LLC ; 2015

In: Nature Communications Vol. 6, No. 1 ( 2015-05-11)

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In: Nature Communications, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2015-05-11)

Abstract: How cells in developing organisms interpret the quantitative information contained in morphogen gradients is an open question. Here we address this question using a novel integrative approach that combines quantitative measurements of morphogen-induced gene expression at single-mRNA resolution with mathematical modelling of the induction process. We focus on the induction of Notch ligands by the LIN-3/EGF morphogen gradient during vulva induction in Caenorhabditis elegans . We show that LIN-3/EGF-induced Notch ligand expression is highly dynamic, exhibiting an abrupt transition from low to high expression. Similar transitions in Notch ligand expression are observed in two highly divergent wild C. elegans isolates. Mathematical modelling and experiments show that this transition is driven by a dynamic increase in the sensitivity of the induced cells to external LIN-3/EGF. Furthermore, this increase in sensitivity is independent of the presence of LIN-3/EGF. Our integrative approach might be useful to study induction by morphogen gradients in other systems.

Type of Medium: Online Resource

ISSN: 2041-1723

URL: Article

DOI: 10.1038/ncomms8053

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2015

detail.hit.zdb_id: 2553671-0

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Effect of Antifreeze Glycoproteins on Organoid Survival during and after Hypothermic Storage

Huelsz-Prince, Guizela ; DeVries, Arthur L. ; Bakker, Huib J. ; [et al.]

MDPI AG ; 2019

In: Biomolecules Vol. 9, No. 3 ( 2019-03-19), p. 110-

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In: Biomolecules, MDPI AG, Vol. 9, No. 3 ( 2019-03-19), p. 110-

Abstract: We study the effect of antifreeze glycoproteins (AFGPs) on the survival of organoids under hypothermic conditions. We find that the survival of organoids in cold conditions depends on their developmental stage. Mature organoids die within 24 h when being stored at 4 °C, while cystic organoids can survive up to 48 h. We find that in the presence of AFGPs, the organoid survival is prolonged up to 72 h, irrespective of their developmental stage. Fluorescence microscopy experiments reveal that the AFGPs predominately localize at the cell surface and cover the cell membranes. Our findings support a mechanism in which the positive effect of AFGPs on cell survival during hypothermic storage involves the direct interaction of AFGPs with the cell membrane. Our research highlights organoids as an attractive multicellular model system for studying the action of AFGPs that bridges the gap between single-cell and whole-organ studies.

Type of Medium: Online Resource

ISSN: 2218-273X

URL: Article

DOI: 10.3390/biom9030110

Language: English

Publisher: MDPI AG

Publication Date: 2019

detail.hit.zdb_id: 2701262-1

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Mother cells control daughter cell proliferation in intestinal organoids to minimize proliferation fluctuations

Huelsz-Prince, Guizela ; Kok, Rutger Nico Ulbe ; Goos, Yvonne ; [et al.]

eLife Sciences Publications, Ltd ; 2022

In: eLife Vol. 11 ( 2022-11-29)

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In: eLife, eLife Sciences Publications, Ltd, Vol. 11 ( 2022-11-29)

Abstract: The vast majority of cells lining our intestine die within three to five days. They are replaced by a small group of stem cells which divide to produce either more stem cells, or cells that stop dividing and transform, or ‘differentiate’, in to mature cells in the intestine. Stem cells must generate the same number of dividing and differentiated cells. If there is even a slight bias and too many stem cells are produced, this can lead to uncontrolled growth, which is the root cause of cancer. In principal, the best way to achieve this balance is for stem cells to always asymmetrically divide in to two distinct cells: one that will continue to divide, and another that will mature in to an adult cell. However, recent research suggests that this process is much more random, with stem cells also dividing symmetrically, either in to two stem cells or two differentiated cells. So, how does the random nature of stem cell divisions not cause the number of dividing cells to fluctuate unpredictably in the intestine? To investigate, Huelsz-Prince et al. studied stem cells in a miniature model of the mouse intestine, known as an organoid, which can be grown outside of the body in a laboratory. All stem cells and their progeny were tracked for over 65 hours using a microscope to see how many dividing and differentiated cells they formed. This revealed that almost all stem cells in the organoid split symmetrically rather than asymmetrically. Huelsz-Prince et al. then developed a computer model of stem cells in the model intestine and tested the impact of changing the proportion of symmetric and asymmetric divisions. The results showed that having more symmetric divisions reduced fluctuations in the number of dividing cells better than high levels of asymmetric divisions. Other organs rely on a similar system to the intestine to replenish their mature cells. Consequently, the finding that symmetric divisions control fluctuations in the number of stem cells may be applicable to other parts of the body. Further testing with human disease samples, such as cells from cancer patients, using the organoid model system may also shed light on how division is disrupted in these conditions.

Type of Medium: Online Resource

ISSN: 2050-084X

URL: Article

DOI: 10.7554/eLife.80682

Language: English

Publisher: eLife Sciences Publications, Ltd

Publication Date: 2022

detail.hit.zdb_id: 2687154-3

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Activation of Extracellular Transglutaminase 2 by Mechanical Force in the Arterial Wall

Huelsz-Prince, Guizela ; Belkin, Alexey M. ; vanBavel, Ed ; [et al.]

S. Karger AG ; 2013

In: Journal of Vascular Research Vol. 50, No. 5 ( 2013), p. 383-395

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In: Journal of Vascular Research, S. Karger AG, Vol. 50, No. 5 ( 2013), p. 383-395

Abstract: Inward remodeling of small arteries occurs after prolonged vasoconstriction, low blood flow, and in several models of hypertension. The cross-linking enzyme, transglutaminases 2 (TG2), is able to induce inward remodeling and stiffening of arteries. The activity of TG2 is dependent on its conformation, which can be open or closed, and on its redox state. Several factors have been shown to be involved in modulating TG2 activity, including Ca 〈 sup 〉 2+ 〈 /sup 〉 and GTP/GDP concentrations, as well as the redox state of the environment. This review introduces the hypothesis that mechanical force could be involved in regulating the activity of TG2 during inward remodeling by promoting its open and reduced active state. Several aspects of TG2, such as its structure and localization, are assessed in order to provide arguments that support the hypothesis. We conclude that a direct activation of TG2 by mechanical force exerted by smooth muscle cells may explain the link between smooth muscle activation and inward remodeling, as observed in several physiological and pathological conditions.

Type of Medium: Online Resource

ISSN: 1018-1172 , 1423-0135

URL: Article

DOI: 10.1159/000354222

Language: English

Publisher: S. Karger AG

Publication Date: 2013

detail.hit.zdb_id: 1482726-8

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Canalization of C. elegans Vulva Induction against Anatomical Variability

Huelsz-Prince, Guizela ; van Zon, Jeroen Sebastiaan

Elsevier BV ; 2017

In: Cell Systems Vol. 4, No. 2 ( 2017-02), p. 219-230.e6

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In: Cell Systems, Elsevier BV, Vol. 4, No. 2 ( 2017-02), p. 219-230.e6

Type of Medium: Online Resource

ISSN: 2405-4712

URL: Article

DOI: 10.1016/j.cels.2017.01.009

Language: English

Publisher: Elsevier BV

Publication Date: 2017

detail.hit.zdb_id: 2854138-8

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Long‐term expanding human airway organoids for disease modeling

Sachs, Norman ; Papaspyropoulos, Angelos ; Zomer‐van Ommen, Domenique D ; [et al.]

EMBO ; 2019

In: The EMBO Journal Vol. 38, No. 4 ( 2019-02-15)

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In: The EMBO Journal, EMBO, Vol. 38, No. 4 ( 2019-02-15)

Type of Medium: Online Resource

ISSN: 0261-4189 , 1460-2075

URL: Article

DOI: 10.15252/embj.2018100300

RVK:

WA 15000

Language: English

Publisher: EMBO

Publication Date: 2019

detail.hit.zdb_id: 1467419-1

detail.hit.zdb_id: 586044-1

SSG: 12

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OrganoidTracker: Efficient cell tracking using machine learning and manual error correction

Kok, Rutger N. U. ; Hebert, Laetitia ; Huelsz-Prince, Guizela ; [et al.]

Public Library of Science (PLoS) ; 2020

In: PLOS ONE Vol. 15, No. 10 ( 2020-10-22), p. e0240802-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 10 ( 2020-10-22), p. e0240802-

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article

DOI: 10.1371/journal.pone.0240802

DOI: 10.1371/journal.pone.0240802.g001

DOI: 10.1371/journal.pone.0240802.g002

DOI: 10.1371/journal.pone.0240802.g003

DOI: 10.1371/journal.pone.0240802.g004

DOI: 10.1371/journal.pone.0240802.g005

DOI: 10.1371/journal.pone.0240802.s001

DOI: 10.1371/journal.pone.0240802.s002

DOI: 10.1371/journal.pone.0240802.s003

DOI: 10.1371/journal.pone.0240802.s004

DOI: 10.1371/journal.pone.0240802.s005

DOI: 10.1371/journal.pone.0240802.s006

DOI: 10.1371/journal.pone.0240802.s007

DOI: 10.1371/journal.pone.0240802.r001

DOI: 10.1371/journal.pone.0240802.r002

DOI: 10.1371/journal.pone.0240802.r003

DOI: 10.1371/journal.pone.0240802.r004

DOI: 10.1371/journal.pone.0240802.r005

DOI: 10.1371/journal.pone.0240802.r006

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2020

detail.hit.zdb_id: 2267670-3

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Organoid cell fate dynamics in space and time

Zheng, Xuan ; Betjes, Max A. ; Ender, Pascal ; [et al.]

American Association for the Advancement of Science (AAAS) ; 2023

In: Science Advances Vol. 9, No. 33 ( 2023-08-18)

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In: Science Advances, American Association for the Advancement of Science (AAAS), Vol. 9, No. 33 ( 2023-08-18)

Abstract: Tracking all cell types in intestinal organoids reveals a commit-then-sort model that upends current differentiation models.

Type of Medium: Online Resource

ISSN: 2375-2548

URL: Article

DOI: 10.1126/sciadv.add6480

Language: English

Publisher: American Association for the Advancement of Science (AAAS)

Publication Date: 2023

detail.hit.zdb_id: 2810933-8

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