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  • 1
    In: Molecules, MDPI AG, Vol. 27, No. 19 ( 2022-09-20), p. 6144-
    Abstract: The NIS synthetase family of enzymes responsible for the biosynthesis of siderophores is increasingly associated with bacterial virulence. Proteins in this class represent outstanding potential drug targets, assuming that basic biochemical and structural characterizations can be completed. Towards this goal, we have mated an improved synthesis of the non-commercial amino acid N-hydroxy-N-succinylcadaverine (HSC, 6) with an isothermal titration calorimetry (ITC) assay that profiles the iterative stages of HSC trimerization and macrocyclization by NIS synthetase DesD from Streptomyces coelicolor. HSC synthesis begins with multigram-scale Gabrielle and tert-butyl N-(benzyloxy)carbamate alkylations of 1-bromo-5-chloropentane following prior literature, but the end-game reported herein has two advantages for greater material throughput: (1) hydrogenolysis of benzyl ether and Cbz blocking groups is best accomplished with Pearlman’s catalyst at 40 psi of H2 and (2) purification of neutral (zwitterionic) HSC is effected by simple flash chromatography over silica gel in MeOH. HSC is subsequently shown to be a substrate for NIS synthetase DesD, which catalyzes three successive amide bond syntheses via adenyl monophosphate ester intermediates. We quantify and present the iterative and overall enzyme kinetic constants associated with formation of the cyclotrimeric siderophore desferrioxamine E (dfoE, 1).
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 2
    Online Resource
    Online Resource
    Wiley ; 2013
    In:  North American Journal of Fisheries Management Vol. 33, No. 1 ( 2013-02), p. 44-55
    In: North American Journal of Fisheries Management, Wiley, Vol. 33, No. 1 ( 2013-02), p. 44-55
    Abstract: This study estimated the economic benefit of recreational fisheries on two trophy crappie Pomoxis spp. fisheries in Mississippi. We accomplished this by estimating economic impacts of angler expenditures and angler willingness‐to‐pay for fishing trips above current expenditures. Anglers spent 91,811 activity days on Sardis Reservoir in 2006 and 46,036 on Grenada Reservoir in 2007. The total economic impacts to the state were estimated at US$5.83 million on Sardis Reservoir and $2.15 million on Grenada Reservoir, supporting 75 and 51 full‐ and part‐time jobs, respectively. Total consumer surplus was $5.57 million and $4.46 million on Sardis and Grenada reservoirs, respectively. Last, we used a model that predicted the effect of average spring water levels on total angling effort to estimate resulting changes in economic benefits. Spring angling effort and associated economic benefits peaked at intermediate water levels and declined at both low and high water levels. Future changes in precipitation patterns in the southeastern United States may require altering reservoir guide curves to maximize economic benefits associated with reservoir fisheries. Received June 25, 2012; accepted October 3, 2012
    Type of Medium: Online Resource
    ISSN: 0275-5947 , 1548-8675
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 2192453-3
    SSG: 21,3
    SSG: 12
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