In:
FEBS Letters, Wiley, Vol. 448, No. 1 ( 1999-04-02), p. 145-148
Abstract:
Substitution of Asn for the conserved Ser 543 in the thumb subdomain of the Taq DNA polymerase large fragment ( Klentaq DNA polymerase) prevents pausing during DNA synthesis and allows the enzyme to circumvent template regions with a complex structure. The mutant enzyme ( KlentaqN DNA polymerase) provides specific PCR amplification and sequencing of difficult templates, e.g. those with a high GC% content or strong secondary structure.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(99)00353-1
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
1460391-3
SSG:
12
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