In:
Pharmacia, Pensoft Publishers, Vol. 67, No. 2 ( 2020-07-31), p. 95-99
Abstract:
Establishment of in vitro cultures from Astragalus glycyphyllos , determination of biomass and analysis of total flavonoids, rutin and camelliaside A were performed. To increase flavonoid production various combinations of plant hormones and light/dark regimen were investigated. Suspension cultures with exogenous quercetin were evaluated for possible increase in flavonoid production. Shoots, calli and suspensions were successfully established. Rutin and camelliaside A were proved in highest amount in shoots. Calli, cultivated on modified G48 medium, with double amount of Ca 2+ and Mg 2+ , achieved higher total flavonoid content (2.37 and 2.03 mg/g DW). Suspensions cultures, cultivated on modified G48 medium with 10, 20 and 30 mg/mL quercetin achieved higher total flavonoid content (0.09, 0.10 and 0.13 mg/mg DW). Biotransformation of quercetin to isoquercitrin was achieved. The highest concentration of isoquercitrin (56.73 ng/mg DW) was observed on suspensions cultures cultivated on modified G48 medium, with 20 mg/mL quercetin.
Type of Medium:
Online Resource
ISSN:
2603-557X
,
0428-0296
DOI:
10.3897/pharmacia.67.e50390
DOI:
10.3897/pharmacia.67.e50390.figure1
DOI:
10.3897/pharmacia.67.e50390.figure2
DOI:
10.3897/pharmacia.67.e50390.figure3
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10.3897/pharmacia.67.e50390.figure4
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10.3897/pharmacia.67.e50390.figure5
DOI:
10.3897/pharmacia.67.e50390.figure6
Language:
Unknown
Publisher:
Pensoft Publishers
Publication Date:
2020
detail.hit.zdb_id:
3010005-7
SSG:
15,3
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