In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2022-8-10), p. e0272799-
Abstract:
Horseshoe crabs are one of the most studied invertebrates due to their remarkable innate immunity mechanism and biological processes. In this work, the proteins of the lipopolysaccharides (LPS)-stimulated and non-stimulated hemocytes of Malaysian Tachypleus gigas were profiled using LC-MS/MS. A total of 154 proteins were identified in both types of samples. Additionally, seventy-seven proteins were commonly found in both conditions, while 52 and 25 proteins were uniquely found in the LPS-stimulated and non-stimulated hemocytes, respectively. ATP-dependent energy-generating proteins such as actins and BLTX actin-related proteins were detected in both stimulated and non-stimulated T . gigas hemocytes, but more of such proteins were found in the former type. Proteins such as tachylectin-2, coagulogen, c-reactive proteins, histones, hemocyanin, and DNA polymerase, which play key roles in the organism’s innate immunity, were differentially expressed in the hemocytes following LPS challenge. In conclusion, the proteins identified in the hemolymph of T . gigas are vital for the organism’s molecular functions, biological processes, and activation of innate immunity.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0272799
DOI:
10.1371/journal.pone.0272799.g001
DOI:
10.1371/journal.pone.0272799.g002
DOI:
10.1371/journal.pone.0272799.g003
DOI:
10.1371/journal.pone.0272799.g004
DOI:
10.1371/journal.pone.0272799.s001
DOI:
10.1371/journal.pone.0272799.s002
DOI:
10.1371/journal.pone.0272799.s003
DOI:
10.1371/journal.pone.0272799.s004
DOI:
10.1371/journal.pone.0272799.s005
DOI:
10.1371/journal.pone.0272799.s006
DOI:
10.1371/journal.pone.0272799.s007
DOI:
10.1371/journal.pone.0272799.s008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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