In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 5 ( 2021-5-13), p. e0251456-
Abstract:
Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. In this study, we found that Pbp1 has a role in cell growth on the medium containing non-fermentable carbon sources. While the pbp1Δ mutant showed a similar growth compared to the wild-type cell on a normal glucose-containing medium, the pbp1Δ mutant showed a slower growth on the medium containing glycerol and lactate. Microarray analyses revealed that expressions of the genes involved in gluconeogenesis, such as PCK1 and FBP1 , and of the genes involved in mitochondrial function, such as COX10 and COX11 , were decreased in the pbp1Δ mutant. Pbp1 regulated the expressions of PCK1 and FBP1 via their promoters, while the expressions of COX10 and COX11 were regulated by Pbp1, not through their promoters. The decreased expressions of COX10 and COX11 in the pbp1Δ mutant were recovered by the loss of Dcp1 decapping enzyme or Xrn1 5’-3’exonuclease. Our results suggest that Pbp1 regulates the expressions of the genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0251456
DOI:
10.1371/journal.pone.0251456.g001
DOI:
10.1371/journal.pone.0251456.g002
DOI:
10.1371/journal.pone.0251456.g003
DOI:
10.1371/journal.pone.0251456.g004
DOI:
10.1371/journal.pone.0251456.g005
DOI:
10.1371/journal.pone.0251456.g006
DOI:
10.1371/journal.pone.0251456.g007
DOI:
10.1371/journal.pone.0251456.g008
DOI:
10.1371/journal.pone.0251456.g009
DOI:
10.1371/journal.pone.0251456.g010
DOI:
10.1371/journal.pone.0251456.g011
DOI:
10.1371/journal.pone.0251456.g012
DOI:
10.1371/journal.pone.0251456.g013
DOI:
10.1371/journal.pone.0251456.g014
DOI:
10.1371/journal.pone.0251456.t001
DOI:
10.1371/journal.pone.0251456.t002
DOI:
10.1371/journal.pone.0251456.t003
DOI:
10.1371/journal.pone.0251456.s001
DOI:
10.1371/journal.pone.0251456.s002
DOI:
10.1371/journal.pone.0251456.s003
DOI:
10.1371/journal.pone.0251456.s004
DOI:
10.1371/journal.pone.0251456.s005
DOI:
10.1371/journal.pone.0251456.s006
DOI:
10.1371/journal.pone.0251456.s007
DOI:
10.1371/journal.pone.0251456.s008
DOI:
10.1371/journal.pone.0251456.s009
DOI:
10.1371/journal.pone.0251456.r001
DOI:
10.1371/journal.pone.0251456.r002
DOI:
10.1371/journal.pone.0251456.r003
DOI:
10.1371/journal.pone.0251456.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Bookmarklink