In:
Frontiers in Immunology, Frontiers Media SA, Vol. 14 ( 2023-9-18)
Abstract:
Streptococcus pneumoniae is one of the main causes of community-acquired infections in the lung alveoli in children and the elderly. Alveolar macrophages (AM) patrol alveoli in homeostasis and under infectious conditions. However, the molecular adaptations of AM upon infections with Streptococcus pneumoniae are incompletely resolved. Methods We used a comparative transcriptomic and proteomic approach to provide novel insights into the cellular mechanism that changes the molecular signature of AM during lung infections. Using a tandem mass spectrometry approach to murine cell-sorted AM, we revealed significant proteomic changes upon lung infection with Streptococcus pneumoniae. Results AM showed a strong neutrophil-associated proteomic signature, such as expression of CD11b, MPO, neutrophil gelatinases, and elastases, which was associated with phagocytosis of recruited neutrophils. Transcriptomic analysis indicated intrinsic expression of CD11b by AM. Moreover, comparative transcriptomic and proteomic profiling identified CD11b as the central molecular hub in AM, which influenced neutrophil recruitment, activation, and migration. Discussion In conclusion, our study provides novel insights into the intrinsic molecular adaptations of AM upon lung infection with Streptococcus pneumoniae and reveals profound alterations critical for effective antimicrobial immunity.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2023.1227191
DOI:
10.3389/fimmu.2023.1227191.s001
DOI:
10.3389/fimmu.2023.1227191.s002
DOI:
10.3389/fimmu.2023.1227191.s003
DOI:
10.3389/fimmu.2023.1227191.s004
DOI:
10.3389/fimmu.2023.1227191.s005
DOI:
10.3389/fimmu.2023.1227191.s006
DOI:
10.3389/fimmu.2023.1227191.s007
DOI:
10.3389/fimmu.2023.1227191.s008
DOI:
10.3389/fimmu.2023.1227191.s009
DOI:
10.3389/fimmu.2023.1227191.s010
DOI:
10.3389/fimmu.2023.1227191.s011
DOI:
10.3389/fimmu.2023.1227191.s012
DOI:
10.3389/fimmu.2023.1227191.s013
DOI:
10.3389/fimmu.2023.1227191.s014
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2606827-8
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