In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 61, No. 4 ( 2011-04-01), p. 956-960
Abstract:
A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31 T , was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis of the isolated bacterium indicated its phylogenetic position within the genus Leucobacter . Binary 16S rRNA gene sequence alignments of the isolated bacterium with the 11 species of the genus recognized at the time of writing revealed sequence similarities of more than 97 % with Leucobacter alluvii (GenBank accession no: AM072820; 99.4 %), Leucobacter iarius (AM040493; 98.2 %), Leucobacter aridicollis (AJ781047; 97.8 %), Leucobacter komagatae (AB007419; 97.4 %), Leucobacter chironomi (EU346911; 97.1 %) and Leucobacter luti (AM072819; 97.1 %). In contrast, DNA–DNA hybridization experiments showed similarity values below 28 % for DNA samples from the most closely related type strains of L. alluvii , L. aridicollis and L. iarius . Protein analysis by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and automated RiboPrinting using the restriction enzyme Pvu II differentiated strain JG 31 T from all type strains of the genus Leucobacter . The dominant fatty acids of the novel isolate were anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 , while the quinone system consisted of menaquinones MK-11, MK-10, MK-9 and MK-8. In a B-type cross-linked peptidoglycan, the cell-wall amino acids were alanine, glycine, threonine, glutamic acid and 2,4-diaminobutyric acid. Strain JG 31 T was able to grow in a medium containing up to 300 mM K 2 CrO 4 and showed cellular aggregation in response to chromate stress. From biochemical and genomic analyses, the new strain is considered to represent a novel species of the genus Leucobacter , for which the name Leucobacter chromiiresistens sp. nov. is proposed. The type strain is strain JG 31 T ( = DSM 22788 T = CCOS 200 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.022780-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2011
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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