In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-24), p. e0246363-
Abstract:
To investigate the role of tannin-enriched extracts of Ecklonia cava (TEE) on the regulation of oxidative balance and laxative activity in chronic constipation, we investigated alterations after exposure to TEE, on constipation phenotypes, muscarinic cholinergic regulation, and oxidative stress responses in the transverse colons of SD rats with loperamide (Lop)-induced constipation. This extract contains high levels of total condensed tannin content (326.5 mg/g), and exhibited high inhibitory activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. TEE treatment induced significant improvements in reactive oxygen species (ROS) production, superoxide dismutase (SOD) expression and nuclear factor erythroid 2-related factor 2 (Nrf2) phosphorylation in primary smooth muscles of rat intestine cells (pRISMCs) and transverse colon of constipation model. Also, Lop+TEE treated groups showed alleviated outcomes for the following: most stool parameters, gastrointestinal transit, and intestine length were remarkably recovered; a similar recovery pattern was observed in the histopathological structure, mucin secretion, water channel expression and gastrointestinal hormones secretion in the transverse colon; expressions of muscarinic acetylcholine receptors M2/M3 (mAChR M2/M3) and their mediators on muscarinic cholinergic regulation were significantly recovered. Taken together, these results provide the first evidence that TEE stimulates oxidative stress modulation and muscarinic cholinergic regulation when exerting its laxative effects in chronic constipation models.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0246363
DOI:
10.1371/journal.pone.0246363.g001
DOI:
10.1371/journal.pone.0246363.g002
DOI:
10.1371/journal.pone.0246363.g003
DOI:
10.1371/journal.pone.0246363.g004
DOI:
10.1371/journal.pone.0246363.g005
DOI:
10.1371/journal.pone.0246363.g006
DOI:
10.1371/journal.pone.0246363.g007
DOI:
10.1371/journal.pone.0246363.g008
DOI:
10.1371/journal.pone.0246363.g009
DOI:
10.1371/journal.pone.0246363.s001
DOI:
10.1371/journal.pone.0246363.r001
DOI:
10.1371/journal.pone.0246363.r002
DOI:
10.1371/journal.pone.0246363.r003
DOI:
10.1371/journal.pone.0246363.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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