In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 114, No. 10 ( 2015-11-28), p. 1560-1568
Abstract:
The present study was conducted to investigate the anti-inflammatory effect of vitamin D both in juvenile Jian carp ( Cyprinus carpio var. Jian) in vivo and in enterocytes in vitro . In primary enterocytes, exposure to 10 mg lipopolysaccharide (LPS)/l increased lactate dehydrogenase activity in the culture medium ( P 〈 0·05) and resulted in a significant loss of cell viability ( P 〈 0·05). LPS exposure increased ( P 〈 0·05) the mRNA expression of pro-inflammatory cytokines (TNF- α , IL-1 β , IL-6 and IL-8), which was decreased by pre-treatment with 1,25-dihydroxyvitamin D (1,25D3) in a dose-dependent manner ( P 〈 0·05). Further results showed that pre-treatment with 1,25D3 down-regulated Toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (Myd88) and NF- κ B p65 mRNA expression ( P 〈 0·05), suggesting potential mechanisms against LPS-induced inflammatory response. In vivo , intraperitoneal injection of LPS significantly increased TNF- α , IL-1 β , IL-6 and IL-8 mRNA expression in the intestine of carp ( P 〈 0·05). Pre-treatment of fish with vitamin D 3 protected the fish intestine from the LPS-induced increase of TNF- α , IL-1 β , IL-6 and IL-8 mainly by downregulating TLR4, Myd88 and NF- κ B p65 mRNA expression ( P 〈 0·05). These observations suggest that vitamin D could inhibit LPS-induced inflammatory response in juvenile Jian carp in vivo and in enterocytes in vitro . The anti-inflammatory effect of vitamin D is mediated at least in part by TLR4-Myd88 signalling pathways in the intestine and enterocytes of juvenile Jian carp.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114515003256
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2015
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
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