In:
MycoKeys, Pensoft Publishers, Vol. 40 ( 2018-10-10), p. 89-110
Abstract:
An extended incubation strategy to culture slow growing members of anaerobic fungi resulted in the isolation of a novel anaerobic fungus from the rumen of a goat after 15 days. The novel genus, represented by type strain G1SC, showed filamentous monocentric thallus development and produced uniflagellate zoospores, hence, showing morphological similarity to the genera Piromyces , Buwchfawromyces , Oontomyces and Pecoramyces . However, strain G1SC showed genetic similarity to the genus Anaeromyces , which, though produces uniflagellate zoospore, also exhibits polycentric thallus development. Moreover, unlike Anaeromyces , strain G1SC did not show hyphal constrictions, instead produced a branched, determinate and anucleate rhizoidal system. This fungus also displayed extensive sporangial variations, both exogenous and endogenous type of development, short and long sporangiophores and produced septate sporangia. G1SC utilised various complex and simple substrates, including rice straw and wheat straw and produced H 2 , CO 2 , formate, acetate, lactate, succinate and ethanol. Phylogenetic analysis, using internal transcribed spacer 1 (ITS1) and D1/D2 domain of large-subunit (LSU) rRNA locus, clearly showed a separate lineage for this strain, near Anaeromyces . The ITS1 based geographical distribution studies indicated detection of environmental sequences similar (93–96%) to this strain from cattle faeces. Based on morphological and molecular characterisation results of strain G1SC, we propose a novel anaerobic fungus Liebetanzomycespolymorphus gen. et sp. nov. , in the phylum Neocallimastigomycota .
Type of Medium:
Online Resource
ISSN:
1314-4049
,
1314-4057
DOI:
10.3897/mycokeys.40.28337
DOI:
10.3897/mycokeys.40.28337.figure1
DOI:
10.3897/mycokeys.40.28337.figure2
DOI:
10.3897/mycokeys.40.28337.figure3
DOI:
10.3897/mycokeys.40.28337.figure4
DOI:
10.3897/mycokeys.40.28337.figure5
DOI:
10.3897/mycokeys.40.28337.figure6
DOI:
10.3897/mycokeys.40.28337.suppl1
DOI:
10.3897/mycokeys.40.28337.suppl2
DOI:
10.3897/mycokeys.40.28337.suppl3
DOI:
10.3897/mycokeys.40.28337.suppl4
Language:
Unknown
Publisher:
Pensoft Publishers
Publication Date:
2018
detail.hit.zdb_id:
2630648-7
Bookmarklink