In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 15 ( 2024-5-2)
Abstract:
Iron- and reactive oxygen species (ROS)-dependent ferroptosis occurs in plant cells. Ca 2+ acts as a conserved key mediator to control plant immune responses. Here, we report a novel role of cytoplasmic Ca 2+ influx regulating ferroptotic cell death in rice immunity using pharmacological approaches. High Ca 2+ influx triggered iron-dependent ROS accumulation, lipid peroxidation, and subsequent hypersensitive response (HR) cell death in rice ( Oryza sativa ). During Magnaporthe oryzae infection, 14 different Ca 2+ influx regulators altered Ca 2+ , ROS and Fe 2+ accumulation, glutathione reductase ( GR ) expression, glutathione (GSH) depletion and lipid peroxidation, leading to ferroptotic cell death in rice. High Ca 2+ levels inhibited the reduction of glutathione isulphide (GSSG) to GSH in vitro . Ca 2+ chelation by ethylene glycol-bis (2-aminoethylether)- N, N, N’, N’ -tetra-acetic acid (EGTA) suppressed apoplastic Ca 2+ influx in rice leaf sheaths during infection. Blocking apoplastic Ca 2+ influx into the cytoplasm by Ca 2+ chelation effectively suppressed Ca 2+ -mediated iron-dependent ROS accumulation and ferroptotic cell death. By contrast, acibenzolar- S -methyl (ASM), a plant defense activator, significantly enhanced Ca 2+ influx, as well as ROS and iron accumulation to trigger ferroptotic cell death in rice. The cytoplasmic Ca 2+ influx through calcium-permeable cation channels, including the putative resistosomes, could mediate iron- and ROS-dependent ferroptotic cell death under reduced GR expression levels in rice immune responses.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2024.1339559
DOI:
10.3389/fpls.2024.1339559.s001
DOI:
10.3389/fpls.2024.1339559.s002
DOI:
10.3389/fpls.2024.1339559.s003
DOI:
10.3389/fpls.2024.1339559.s004
DOI:
10.3389/fpls.2024.1339559.s005
DOI:
10.3389/fpls.2024.1339559.s006
DOI:
10.3389/fpls.2024.1339559.s007
DOI:
10.3389/fpls.2024.1339559.s008
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2024
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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