In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_12 ( 2015-12-01), p. 4823-4829
Abstract:
A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse ( Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16S rRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus , with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis , 95.6 % sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 % sequence similarity to the type strain of Streptobacillus felis . The clear differentiation of strain AHL 370-1 T from Streptobacillus moniliformis , Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB , groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1 T and Streptobacillus moniliformis DSM 12112 T . Physiological data confirmed the allocation of strain AHL 370-1 T to the family Leptotrichiaceae , considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus , isolate AHL 370-1 T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1 T . On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL 370-1 T ( = CCUG 12425 T = DSM 100026 T = CCM 8593 T = EF 12425 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.000654
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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