In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 6 ( 2023-6-28), p. e3002164-
Abstract:
A defining property of circadian clocks is temperature compensation, characterized by the resilience of their near 24-hour free-running periods against changes in environmental temperature within the physiological range. While temperature compensation is evolutionary conserved across different taxa of life and has been studied within many model organisms, its molecular underpinnings remain elusive. Posttranscriptional regulations such as temperature-sensitive alternative splicing or phosphorylation have been described as underlying reactions. Here, we show that knockdown of cleavage and polyadenylation specificity factor subunit 6 ( CPSF6 ), a key regulator of 3′-end cleavage and polyadenylation, significantly alters circadian temperature compensation in human U-2 OS cells. We apply a combination of 3′-end-RNA-seq and mass spectrometry–based proteomics to globally quantify changes in 3′ UTR length as well as gene and protein expression between wild-type and CPSF6 knockdown cells and their dependency on temperature. Since changes in temperature compensation behavior should be reflected in alterations of temperature responses within one or all of the 3 regulatory layers, we statistically assess differential responses upon changes in ambient temperature between wild-type and CPSF6 knockdown cells. By this means, we reveal candidate genes underlying circadian temperature compensation, including eukaryotic translation initiation factor 2 subunit 1 ( EIF2S1 ).
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002164
DOI:
10.1371/journal.pbio.3002164.g001
DOI:
10.1371/journal.pbio.3002164.g002
DOI:
10.1371/journal.pbio.3002164.g003
DOI:
10.1371/journal.pbio.3002164.g004
DOI:
10.1371/journal.pbio.3002164.g005
DOI:
10.1371/journal.pbio.3002164.s001
DOI:
10.1371/journal.pbio.3002164.s002
DOI:
10.1371/journal.pbio.3002164.s003
DOI:
10.1371/journal.pbio.3002164.s004
DOI:
10.1371/journal.pbio.3002164.s005
DOI:
10.1371/journal.pbio.3002164.s006
DOI:
10.1371/journal.pbio.3002164.s007
DOI:
10.1371/journal.pbio.3002164.s008
DOI:
10.1371/journal.pbio.3002164.s009
DOI:
10.1371/journal.pbio.3002164.s010
DOI:
10.1371/journal.pbio.3002164.s011
DOI:
10.1371/journal.pbio.3002164.s012
DOI:
10.1371/journal.pbio.3002164.s013
DOI:
10.1371/journal.pbio.3002164.s014
DOI:
10.1371/journal.pbio.3002164.s015
DOI:
10.1371/journal.pbio.3002164.s016
DOI:
10.1371/journal.pbio.3002164.s017
DOI:
10.1371/journal.pbio.3002164.s018
DOI:
10.1371/journal.pbio.3002164.s019
DOI:
10.1371/journal.pbio.3002164.s020
DOI:
10.1371/journal.pbio.3002164.s021
DOI:
10.1371/journal.pbio.3002164.s022
DOI:
10.1371/journal.pbio.3002164.s023
DOI:
10.1371/journal.pbio.3002164.s024
DOI:
10.1371/journal.pbio.3002164.s025
DOI:
10.1371/journal.pbio.3002164.s026
DOI:
10.1371/journal.pbio.3002164.s027
DOI:
10.1371/journal.pbio.3002164.s028
DOI:
10.1371/journal.pbio.3002164.s029
DOI:
10.1371/journal.pbio.3002164.s030
DOI:
10.1371/journal.pbio.3002164.s031
DOI:
10.1371/journal.pbio.3002164.s032
DOI:
10.1371/journal.pbio.3002164.s033
DOI:
10.1371/journal.pbio.3002164.s034
DOI:
10.1371/journal.pbio.3002164.r001
DOI:
10.1371/journal.pbio.3002164.r002
DOI:
10.1371/journal.pbio.3002164.r003
DOI:
10.1371/journal.pbio.3002164.r004
DOI:
10.1371/journal.pbio.3002164.r005
DOI:
10.1371/journal.pbio.3002164.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
Bookmarklink