In:
Angewandte Chemie, Wiley, Vol. 131, No. 29 ( 2019-07-15), p. 10022-10027
Abstract:
Ca 2+ handling by mitochondria is crucial for cell life and the direct measure of mitochondrial Ca 2+ concentration in living cells is of pivotal interest. Genetically‐encoded indicators greatly facilitated this task, however they require demanding delivery procedures. On the other hand, existing mitochondria‐targeted synthetic Ca 2+ indicators are plagued by several drawbacks, for example, non‐specific localization, leakage, toxicity. Here we report the synthesis and characterization of a new fluorescent Ca 2+ sensor, named mt‐fura‐2, obtained by coupling two triphenylphosphonium cations to the molecular backbone of the ratiometric Ca 2+ indicator fura‐2. Mt‐fura‐2 binds Ca 2+ with a dissociation constant of ≈1.5 μ m in vitro. When loaded in different cell types as acetoxymethyl ester, the probe shows proper mitochondrial localization and accurately measures matrix [Ca 2+ ] variations, proving its superiority over available dyes. We describe the synthesis, characterization and application of mt‐fura‐2 to cell types where the delivery of genetically‐encoded indicators is troublesome.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v131.29
DOI:
10.1002/ange.201902272
Language:
English
Publisher:
Wiley
Publication Date:
2019
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