In:
Chemistry & Biodiversity, Wiley, Vol. 14, No. 8 ( 2017-08)
Abstract:
Seeds of Hypericum perforatum and H. tetrapterum were extracted with dichloromethane and methanol and investigated by chromatographic and mass spectrometric methods. Both species yielded a fatty oil fraction amounting to 30.5% and 18.0% of the seed weight, respectively. Linoleic acid (C18:2n‐6) was shown to be the predominant fatty acid constituent. Moreover, xanthone derivatives, i.e . tetrahydroxyxanthones ( THX ), xanthone‐glycosides and xanthone‐sulfonates, were assigned in methanolic extracts. For structure elucidation, one representative xanthone, namely 1,3,6,7‐ THX , was synthesized and analyzed via HPLC ‐ DAD / MS n and GC / MS . Total THX contents were quantitated applying a validated HPLC ‐ DAD method, resulting in 1.25 g/kg ( H. perforatum ) and 0.27 g/kg ( H. tetrapterum ), respectively. Moreover, the free radical scavenging capacity of the methanol extracts was tested using the DPPH antioxidant assay. Both, H. perforatum ( IC 50 = 8.7 mg/l) and 1,3,6,7‐ THX ( IC 50 = 3.0 mg/l), exhibited good DPPH free radical scavenging activity compared to Trolox ( IC 50 = 6.6 mg/l).
Type of Medium:
Online Resource
ISSN:
1612-1872
,
1612-1880
DOI:
10.1002/cbdv.201700100
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2139001-0
SSG:
12
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