In:
Acta Veterinaria Brno, University of Veterinary Sciences Brno, Vol. 89, No. 4 ( 2020), p. 401-411
Abstract:
The absence of acquired resistance to antimicrobials has become an important criterion in evaluation of the biosafety of lactobacilli used as industrial starter or probiotic cultures. The aim of this study was to assess antibiotic resistance in starter and non-starter lactobacilli of food origin. Minimal inhibitory concentrations of ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, streptomycin, tetracycline and vancomycin were established in 81 strains of lactobacilli ( L. acidophilus , L. animalis , L. brevis , L. curvatus , L. delbrueckii , L. fermentum , L. helveticus , L. paracasei , L. plantarum , L. rhamnosus and L. sakei ) by the microdilution method. The strains were classified as susceptible or resistant to antimicrobials based on the cut-off values according to the EFSA guideline. Sixty-two strains (77% food isolates, 76% starter or adjunct cultures) were resistant to at least one antimicrobial agent (the most frequently to aminoglycosides). Adjunct cultures showed a higher antibiotic resistance (80%) than starters (60%). Four multiresistant strains (3 food isolates, 1 adjunct culture) were analyzed by whole genome sequencing. One potentially transferable aad E gene (responsible for streptomycin resistance) was detected only in one multi-drug resistant strain of L. animalis originating from an adjunct culture. Thus, there is a risk of horizontal transmission of this gene. It is necessary to eliminate such strains from use in the food industry. This study provides relevant data concerning the use of lactobacilli in safe food production. To ensure food safety, detailed characterization of resistance to antimicrobials is necessary not only in starter strains but also in non-starter lactic acid bacteria isolated from food products.
Type of Medium:
Online Resource
ISSN:
0001-7213
,
1801-7576
DOI:
10.2754/avb202089040401
Language:
English
Publisher:
University of Veterinary Sciences Brno
Publication Date:
2020
detail.hit.zdb_id:
2106644-9
SSG:
22
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