In:
FEBS Letters, Wiley, Vol. 467, No. 2-3 ( 2000-02-11), p. 341-347
Abstract:
The data presented implicate a GATA binding site in the transcriptional regulation of 15‐lipoxygenase‐1 (15‐LO‐1) gene expression in human colorectal carcinoma Caco‐2 cells. High expression of GATA‐6 mRNA and protein was observed, while GATA‐4 mRNA was expressed at a very low level in Caco‐2 cells. The expression of GATA‐6 was down‐regulated, while 15‐LO‐1 expression was dramatically up‐regulated after treatment with sodium butyrate (NaBT). A study using an electrophoretic mobility shift assay indicated that a GATA binding site of the 15‐LO‐1 promoter region binds to GATA proteins present in both undifferentiated and, to a lesser extent, NaBT‐treated (differentiated) Caco‐2 cells. Moreover, that DNA binding shift band was disrupted after the addition of GATA‐6 antibody in a supershift assay in the absence of NaBT, suggesting that GATA‐6 is bound to the GATA binding site of the 15‐LO‐1 promoter in undifferentiated cells. In contrast, the addition of GATA‐6 antibody did not affect the DNA binding ability in NaBT‐induced differentiated cells. On the other hand, mutation of the GATA site of the 15‐LO‐1 promoter decreased the transactivation of the 15‐LO‐1 promoter as measured by luciferase activity in both FBS and NaBT cultured cells, indicating an unknown GATA binding protein to up‐regulate 15‐LO‐1 expression. These implicate the GATA site at −240 of the proximal region of the 15‐LO‐1 promoter in the basic transcription of 15‐LO‐1 gene expression in Caco‐2 cells, with GATA‐6 acting to repress 15‐LO‐1 expression.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(00)01155-8
Language:
English
Publisher:
Wiley
Publication Date:
2000
detail.hit.zdb_id:
1460391-3
SSG:
12
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