In:
Journal of Basic Microbiology, Wiley, Vol. 60, No. 7 ( 2020-07), p. 574-584
Abstract:
The P‐type ATPase CrpA is an important Cu 2+ /Cd 2+ pump in the Aspergilli, significantly contributing to the heavy metal stress tolerance of these ascomycetous fungi. As expected, the deletion of crpA resulted in Cu 2+ /Cd 2+ ‐sensitive phenotypes in Aspergillus nidulans on stress agar plates inoculated with conidia. Nevertheless, paradoxical growth stimulations were observed with the Δ crpA strain in both standard Cu 2+ stress agar plate experiments and cellophane colony harvest (CCH) cultures, when exposed to Cd 2+ . These observations reflect efficient compensatory mechanisms for the loss of CrpA operating under these experimental conditions. It is remarkable that the Δ crpA strain showed a 2.7 times higher Cd biosorption capacity in CCH cultures, which may facilitate the development of new, fungal biomass‐based bioremediation technologies to extract harmful Cd 2+ ions from the environment. The nullification of crpA also significantly changed the spatial distribution of Cu and Cd in CCH cultures, as demonstrated by the combined particle‐induced X‐ray emission and scanning transmission ion microscopy technique. Most important, the centers of gravity for Cu and Cd accumulations of the Δ crpA colonies shifted toward the older regions as compared with wild‐type surface cultures.
Type of Medium:
Online Resource
ISSN:
0233-111X
,
1521-4028
DOI:
10.1002/jobm.202000112
Language:
English
Publisher:
Wiley
Publication Date:
2020
detail.hit.zdb_id:
1480967-9
detail.hit.zdb_id:
632513-0
detail.hit.zdb_id:
203025-1
SSG:
12
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