In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 1 ( 2023-1-20), p. e0280583-
Abstract:
Campylobacter jejuni is a major cause of bacterial diarrhea worldwide and associated with numerous sequela, including Guillain-Barré Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. C . jejuni is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (CPS). The genes responsible for the biosynthesis of the phase variable CPS is located in the hypervariable region of C . jejuni genome which has been used to develop multiplex PCR to classify CPS types based on the Penner serotypes. However, there still are non-typable CPS C . jejuni by the current multiplex PCR scheme. The application of the next generation sequencing and whole genome analysis software were used for the identification of novel capsule biosynthesis of C . jejuni isolates. Unique PCR primers were designed to identify these new capsule biosynthesis loci. The designed primers sets were combined in a new multiplex mix called epsilon. The unique sequences provide an additional information of the biosynthesis loci responsible for some of the common CPS sugars/residues such as heptose, deoxtyheptose and MeOPN among C . jejuni in this new group of CPS multiplex assay. This new primer complements the current C . jejuni multiplex capsule typing system and will help in identifying previously untypeable capsule locus of C . jejuni isolates.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0280583
DOI:
10.1371/journal.pone.0280583.g001
DOI:
10.1371/journal.pone.0280583.g002
DOI:
10.1371/journal.pone.0280583.g003
DOI:
10.1371/journal.pone.0280583.t001
DOI:
10.1371/journal.pone.0280583.t002
DOI:
10.1371/journal.pone.0280583.s001
DOI:
10.1371/journal.pone.0280583.s002
DOI:
10.1371/journal.pone.0280583.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
Bookmarklink