In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 74, No. 19_Supplement ( 2014-10-01), p. 560-560
Abstract:
Solid pseudopapillary neoplasm (SPN), is an uncommon pancreatic tumor with distinct clinicopathologic features. SPNs are characterized by mutation in exon 3 of CTNNB1. However, little is known about SPN tumorigenesis but CTNNB1 mutation. In order to identify the characteristic gene expression patterns of SPN, we performed proteome analysis of SPN and compared to gene expression data. We analyzed pooled SPNs and pooled non-neoplastic pancreas tissues using LTQ-Orbitrap. In total, we identified 862 (444 up-regulated and 418 down-regulated) proteins showed differential expression in SPN compared to non-neoplastic pancreas. We compared proteome profiling compared to mRNA expression of SPNs, and then selected 544 significant proteins presented the same expressional tendency. Many of the overexpressed proteins are related to the activated signaling pathways of SPN consistently with our previous study. Specifically, DKK4 (6.1-fold), APC (1.5-fold), RUVBL1 (1.4-fold) and β-catenin (1.3-fold) involved in Wnt signaling were up-regulated in protein level of SPNs compared to non-neoplastic pancreas. Besides Wnt signaling, we detected several up-regulated proteins including FUS and NONO. The interaction of β-catenin with FUS was reported that involved in the regulation of pre-mRNA splicing. We identified that FUS and NONO interacted directly with β-catenin by immunoprecipitation in β -catenin active cell lines (SW480, HCT116). We found that molecules involved in glycolysis, metabolism and sumoylation were activated in SPNs; HK1 (3.2-fold), PKM2 (3.3-fold), ENO2 (2.1-fold), PDHA (1.5-fold) and SUMO2 (1.6-fold). We also detected that several proteins involved in epithelial-mesenchymal transition were up or down-regulated including CTSB (3.98-fold), VIM (1.98-fold) and JUP (-2.29-fold). Our proteomic profiling study of SPNs provides 1) SPN-specific molecular mechanism for solid-pseudopapillary neoplasm tumorigenesis similar with mRNA expression pattern including Wnt signaling, 2) SPN-specific epithelial-mesenchymal transition, and 3) a metabolism is activated in SPNs. These findings can be used for an early diagnosis biomarker development or molecular therapeutic target. Citation Format: Minhee Park, Minhee Cho, SeongJu Yoon, Hoguen Kim. Distinct protein expression patterns of solid pseudopapillary neoplasm of pancreas. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 560. doi:10.1158/1538-7445.AM2014-560
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2014-560
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2014
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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