In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-04-19)
Abstract:
Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The Arabidopsis genome encodes two groups of 14-3-3s, one of which – epsilon – is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport-related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.24336.001
DOI:
10.7554/eLife.24336.002
DOI:
10.7554/eLife.24336.003
DOI:
10.7554/eLife.24336.004
DOI:
10.7554/eLife.24336.005
DOI:
10.7554/eLife.24336.006
DOI:
10.7554/eLife.24336.007
DOI:
10.7554/eLife.24336.008
DOI:
10.7554/eLife.24336.009
DOI:
10.7554/eLife.24336.010
DOI:
10.7554/eLife.24336.011
DOI:
10.7554/eLife.24336.012
DOI:
10.7554/eLife.24336.013
DOI:
10.7554/eLife.24336.014
DOI:
10.7554/eLife.24336.015
DOI:
10.7554/eLife.24336.016
DOI:
10.7554/eLife.24336.017
DOI:
10.7554/eLife.24336.018
DOI:
10.7554/eLife.24336.019
DOI:
10.7554/eLife.24336.020
DOI:
10.7554/eLife.24336.021
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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