In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-1), p. e0268579-
Abstract:
Aneuploidy and chromosomal instability are both commonly found in cancer. Chromosomal instability leads to karyotype heterogeneity in tumors and is associated with therapy resistance, metastasis and poor prognosis. It has been hypothesized that aneuploidy per se is sufficient to drive CIN, however due to limited models and heterogenous results, it has remained controversial which aspects of aneuploidy can drive CIN. In this study we systematically tested the impact of different types of aneuploidies on the induction of CIN. We generated a plethora of isogenic aneuploid clones harboring whole chromosome or segmental aneuploidies in human p53-deficient RPE-1 cells. We observed increased segregation errors in cells harboring trisomies that strongly correlated to the number of gained genes. Strikingly, we found that clones harboring only monosomies do not induce a CIN phenotype. Finally, we found that an initial chromosome breakage event and subsequent fusion can instigate breakage-fusion-bridge cycles. By investigating the impact of monosomies, trisomies and segmental aneuploidies on chromosomal instability we further deciphered the complex relationship between aneuploidy and CIN.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0268579
DOI:
10.1371/journal.pone.0268579.g001
DOI:
10.1371/journal.pone.0268579.g002
DOI:
10.1371/journal.pone.0268579.g003
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10.1371/journal.pone.0268579.g004
DOI:
10.1371/journal.pone.0268579.g005
DOI:
10.1371/journal.pone.0268579.s001
DOI:
10.1371/journal.pone.0268579.s002
DOI:
10.1371/journal.pone.0268579.s003
DOI:
10.1371/journal.pone.0268579.s004
DOI:
10.1371/journal.pone.0268579.s005
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10.1371/journal.pone.0268579.s006
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10.1371/journal.pone.0268579.s007
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10.1371/journal.pone.0268579.s008
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10.1371/journal.pone.0268579.s009
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10.1371/journal.pone.0268579.s010
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10.1371/journal.pone.0268579.s011
DOI:
10.1371/journal.pone.0268579.s012
DOI:
10.1371/journal.pone.0268579.s013
DOI:
10.1371/journal.pone.0268579.s014
DOI:
10.1371/journal.pone.0268579.r001
DOI:
10.1371/journal.pone.0268579.r002
DOI:
10.1371/journal.pone.0268579.r003
DOI:
10.1371/journal.pone.0268579.r004
DOI:
10.1371/journal.pone.0268579.r005
DOI:
10.1371/journal.pone.0268579.r006
DOI:
10.1371/journal.pone.0268579.r007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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