In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2023-4-26), p. e0284852-
Abstract:
In a retrospective and exploratory case-control study, we examined the genetic association of two common polymorphisms in the 3’ untranslated region (UTR) of DICER1 (rs3742330) and DROSHA (rs10719) genes in primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG), and its related clinical phenotypes in a Saudi cohort. Methods DNA genotyping was performed using TaqMan real-time PCR assays in 500 participants, including 152 POAG, 102 PACG, and 246 non-glaucomatous controls. Statistical analyses were performed to examine the association(s). Results Allele and genotype frequency of rs3742330 and rs10719 did not vary significantly in POAG and PACG compared to controls. No significant deviation was observed from Hardy-Weinberg Equilibrium ( p 〉 0.05). Gender stratification revealed no significant allelic/genotype association with glaucoma types. Also, these polymorphisms showed no significant genotype effect on clinical markers such as intraocular pressure, cup/disc ratio, and the number of antiglaucoma medications. Logistic regression showed no effect of age, sex, rs3742330, and rs10719 genotypes on the risk of disease outcome. We also examined a combined allelic effect of rs3742330 (A 〉 G) and rs10719 (A 〉 G). However, none of the allelic combinations significantly affected POAG and PACG. Conclusions The 3’ UTR polymorphisms rs3742330 and rs10719 of DICER1 and DROSHA genes are not associated with POAG and PACG or its related glaucoma indices in this Middle-Eastern cohort of Saudi Arab ethnicity. However, there is a need to validate the results on a broader population and other ethnicities.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0284852
DOI:
10.1371/journal.pone.0284852.g001
DOI:
10.1371/journal.pone.0284852.g002
DOI:
10.1371/journal.pone.0284852.t001
DOI:
10.1371/journal.pone.0284852.t002
DOI:
10.1371/journal.pone.0284852.t003
DOI:
10.1371/journal.pone.0284852.t004
DOI:
10.1371/journal.pone.0284852.t005
DOI:
10.1371/journal.pone.0284852.s001
DOI:
10.1371/journal.pone.0284852.s002
DOI:
10.1371/journal.pone.0284852.s003
DOI:
10.1371/journal.pone.0284852.s004
DOI:
10.1371/journal.pone.0284852.s005
DOI:
10.1371/journal.pone.0284852.s006
DOI:
10.1371/journal.pone.0284852.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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