In:
Radiology and Oncology, Walter de Gruyter GmbH, Vol. 51, No. 3 ( 2017-6-14), p. 307-316
Abstract:
To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on 131 I-Radioiodine (RAI)-induced salivary gland damage in rats. Materials and Methods Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically. Results We observed the homing of ADMSC in salivary glands at 1 st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner ( P 〈 0.05). Intragroup comparisons with LM at 1 st and 6 th months revealed statistically significant improvements in Group 6 ( P 〈 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1 st month and Group 6 in 6 th month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1 st month, and an improvement in damage in 6 th month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) ( P = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) ( P = 0. 01) at 1 st month, for G/BGmax ( P = 0.01), Smax ( P = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) ( P = 0.03) at 6 th month. 1 st and 6 th month scans showed differences for Smax and G/BGmax ( P = 0.04), but not for Tmax/Tmin ( p 〉 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups. Conclusions Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.
Type of Medium:
Online Resource
ISSN:
1581-3207
DOI:
10.1515/raon-2017-0022
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
2017
detail.hit.zdb_id:
2134813-3
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