In:
Mediators of Inflammation, Wiley, Vol. 2005, No. 6 ( 2005-01), p. 343-348
Abstract:
A massive accumulation of inflammatory cells
in synovial tissues is a major pathological feature of rheumatoid arthritis (RA). Neutrophiles dominate synovial fluid while
rheumatoid synovium is infiltrated with mononuclear cells. Mechanisms regulating influx of particular subpopulations of
leukocytes into articular cavity and synovium compartment are not completely defined. An increasing amount of data supports a
crucial role of a C‐C chemokine RANTES in the RA pathogenesis. Our objective is to evaluate chemotactic activity for
neutrophils (NCA), lymphocytes (LCA), and monocytes (MoCA) in SFs obtained from patients with RA and osteoarthritis (OA). We also
aimed to characterise the relation between chemotactic activity, RANTES, and percentage distribution of leukocytes in SF. SFs from
11 patients with RA and 6 with OA were included in the study. Modified microchamber Boyden method was employed to assess
chemotactic activity. Cytological and biochemical analysis of SF was performed. RANTES was measured with ELISA. Rheumatoid SFs were
rich in cells with predominance of neutrophiles while osteoarthritic fluids were lymphocytic. RA SFs were also
characterised by increased lactoferrin level. Both NCA and LCA were higher in SF from patients with RA (62 ± 12 and 24 ± 6
cells/HPF, resp) as compared to patients with OA (23 ± 6; P 〈 .05
and 6 ± 2 cells/HPF; P 〈 0.05). The chemoattractive
effect of RA SF was more pronounced on neutrophiles than on lymphocytes. RA SF expressed high RANTES levels (145 ± 36pg/mL), while OA SF was characterised by only trace amount
of this chemokine (2 ± 1 pg/mL). We found positive correlation of RANTES with chemotactic activity for mononuclear
cells (LCA + MoCA; R = 0.61; P 〈 .05). Surprisingly,
RANTES correlated also positively with neutrophiles number ( R = 0.77; P 〈 0.001). Rheumatoid SF possesses strong chemotactic
potency for leukocytes. RANTES is overexpressed in RA SF and is a potential mediator influencing intensity and composition of
cellular infiltration in joints affected with inflammatory arthritis.
Type of Medium:
Online Resource
ISSN:
0962-9351
,
1466-1861
Language:
English
Publisher:
Wiley
Publication Date:
2005
detail.hit.zdb_id:
1137605-3
detail.hit.zdb_id:
2008065-7
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