In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-8), p. e0271137-
Abstract:
Sepsis is a diagnostic and therapeutic challenge and is associated with morbidity and a high risk of death. Metabolomic and lipidomic profiling in sepsis can identify alterations in metabolism and might provide useful insights into the dysregulated host response to infection, but investigations in dogs are limited. We aimed to use untargeted metabolomics and lipidomics to characterize metabolic pathways in dogs with sepsis to identify therapeutic targets and potential diagnostic and prognostic biomarkers. In this prospective observational cohort study, we examined the plasma metabolomes and lipidomes of 20 healthy control dogs and compared them with those of 21 client-owned dogs with sepsis. Patient data including signalment, physical exam findings, clinicopathologic data and clinical outcome were recorded. Metabolites were identified using an untargeted mass spectrometry approach and pathway analysis identified multiple enriched metabolic pathways including pyruvaldehyde degradation; ketone body metabolism; the glucose-alanine cycle; vitamin-K metabolism; arginine and betaine metabolism; the biosynthesis of various amino acid classes including the aromatic amino acids; branched chain amino acids; and metabolism of glutamine/glutamate and the glycerophospholipid phosphatidylethanolamine. Metabolites were identified with high discriminant abilities between groups which could serve as potential biomarkers of sepsis including 13,14-Dihydro-15-keto Prostaglandin A 2 ; 12(13)-DiHOME (12,13-dihydroxy-9Z-octadecenoic acid); and 9-HpODE (9-Hydroxyoctadecadienoic acid). Metabolites with higher abundance in samples from nonsurvivors than survivors included 3-(2-hydroxyethyl) indole, indoxyl sulfate and xanthurenic acid. Untargeted lipidomic profiling revealed multiple sphingomyelin species (SM(d34:0)+H; SM(d36:0)+H; SM(d34:0)+HCOO; and SM(d34:1D3)+HCOO); lysophosphatidylcholine molecules (LPC(18:2)+H) and lipophosphoserine molecules (LPS(20:4)+H) that were discriminating for dogs with sepsis. These biomarkers could aid in the diagnosis of dogs with sepsis, provide prognostic information, or act as potential therapeutic targets.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0271137
DOI:
10.1371/journal.pone.0271137.g001
DOI:
10.1371/journal.pone.0271137.g002
DOI:
10.1371/journal.pone.0271137.g003
DOI:
10.1371/journal.pone.0271137.g004
DOI:
10.1371/journal.pone.0271137.g005
DOI:
10.1371/journal.pone.0271137.g006
DOI:
10.1371/journal.pone.0271137.g007
DOI:
10.1371/journal.pone.0271137.g008
DOI:
10.1371/journal.pone.0271137.g009
DOI:
10.1371/journal.pone.0271137.t001
DOI:
10.1371/journal.pone.0271137.t002
DOI:
10.1371/journal.pone.0271137.t003
DOI:
10.1371/journal.pone.0271137.t004
DOI:
10.1371/journal.pone.0271137.t005
DOI:
10.1371/journal.pone.0271137.t006
DOI:
10.1371/journal.pone.0271137.s001
DOI:
10.1371/journal.pone.0271137.s002
DOI:
10.1371/journal.pone.0271137.s003
DOI:
10.1371/journal.pone.0271137.s004
DOI:
10.1371/journal.pone.0271137.s005
DOI:
10.1371/journal.pone.0271137.s006
DOI:
10.1371/journal.pone.0271137.r001
DOI:
10.1371/journal.pone.0271137.r002
DOI:
10.1371/journal.pone.0271137.r003
DOI:
10.1371/journal.pone.0271137.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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