In:
Physiological Research, Institute of Physiology of the Czech Academy of Sciences
Abstract:
The aim of this study was to evaluate macroscopically,
histologically and immunohistochemically the quality of newly formed tissue in iatrogenic defects of articular cartilage of the
femur condyle in miniature pigs treated with the clinically used method of microfractures in comparison with the transplantation
of a combination of a composite scaffold with allogeneic mesenchymal stem cells (MSCs) or the composite scaffold alone.
The newly formed cartilaginous tissue filling the defects of articular cartilage after transplantation of the scaffold with MSCs
(Group A) had in 60 % of cases a macroscopically smooth surface. In all lesions after the transplantation of the scaffold
alone (Group B) or after the method of microfractures (Group C), erosions/fissures or osteophytes were found on the surface. The
results of histological and immunohistochemical examination using the modified scoring system according to O’Driscoll were as
follows: 14.7±3.82 points after transplantations of the scaffold with MSCs (Group A); 5.3±2.88 points after transplantations of
the scaffold alone (Group B); and 5.2±0.64 points after treatment with microfractures (Group C). The O’Driscoll score in animals of
Group A was significantly higher than in animals of Group B or Group C (p 〈 0.0005 both). No significant difference was found in
the O’Driscoll score between Groups B and C. The treatment of iatrogenic lesions of the articular cartilage surface on the
condyles of femur in miniature pigs using transplantation of MSCs in the composite scaffold led to the filling of defects by a tissue
of the appearance of hyaline cartilage. Lesions treated by implantation of the scaffold alone or by the method of
microfractures were filled with fibrous cartilage with worse macroscopic, histological and immunohistochemial indicators.
Type of Medium:
Online Resource
ISSN:
1802-9973
,
0862-8408
DOI:
10.33549/physiolres.931725
Language:
English
Publisher:
Institute of Physiology of the Czech Academy of Sciences
Publication Date:
2010
detail.hit.zdb_id:
2100162-5
SSG:
12
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