In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 70, No. 8_Supplement ( 2010-04-15), p. 4209-4209
Abstract:
Ultrasonic aspirators are commonly used to resect brain tumors because they allow safe, rapid and accurate removal of dissected tissue. However, the tissue fragments removed by ultrasonic aspirators are used surprisingly little in daily diagnostics and in clinical research. Due to the increasing need for vital human tumor tissue for these purposes, which today require multidisciplinary approaches, the aim of this study was to investigate whether tissue fragments obtained by ultrasonic aspiration could be cultured as organotypic multicellular spheroids. These spheroids have earlier been shown to represent an important alternative to commercially available cell lines having multiple mutations and phenotypic changes. Fragments obtained by ultrasonic aspiration (sonocut) from one anaplastic astrocytoma and seven glioblastomas and tumor biopsy tissue from four of these glioblastomas were included in the present study. Sonocut and tumor biopsy tissue were mechanically dissociated into small tissue fragments and cultured in a serum containing medium on agar-coated plates. If excess tissue was available the fragments were also cultured in a serum-free medium. After 12-18 days of culturing the tissue fragments had formed spheroids which were fixated and paraffin embedded. Hematoxylin eosin staining was performed in order to investigate the viability and morphology of the spheroids. Part of the material was stained immunohistochemically for the astrocytic marker GFAP and the blood vessel markers VWF, CD31 and CD34. In the present study all sonocut cultures contained viable spheroids, whereas one of the cultures obtained from tumor biopsy tissue did not. The spheroids were able to survive and proliferate under both serum and serum-free conditions. The spheroids from both sonocut and tumor biopsy tissue contained malignant traits including high cellularity, cellular pleomorphism, mitoses and necrosis. Some spheroids consisted solely of GFAP positive tumor cells, while others contained preserved blood vessels confirmed by VWF, CD31 and CD34 immunohistochemistry. In conclusion, we found that it was possible to obtain viable spheroids from sonocut-material under both serum and serum-free conditions. Note: This abstract was not presented at the AACR 101st Annual Meeting 2010 because the presenter was unable to attend. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 4209.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM10-4209
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2010
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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