In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 6, No. 1 ( 2016-10-26)
Abstract:
Lung cancer is the number one cause of cancer-related deaths worldwide. DNA methylation is an epigenetic mechanism that regulates gene expression, and disease-specific methylation changes can be targeted as biomarkers. We have compared the genome-wide methylation pattern in tumor and tumor-adjacent normal lung tissue from four lung adenocarcinoma (LAC) patients using DNA methylation microarrays and identified 74 differentially methylated regions (DMRs). Eighteen DMRs were selected for validation in a cohort comprising primary tumors from 52 LAC patients and tumor-adjacent normal lung tissue from 32 patients by methylation-sensitive high resolution melting (MS-HRM) analysis. Significant increases in methylation were confirmed for 15 DMRs associated with the genes and genomic regions: OSR1 , SIM1 , GHSR , OTX2 , LOC648987 , HIST1H3E , HIST1H3G/HIST1H2BI , HIST1H2AJ/HIST1H2BM, HOXD10 , HOXD3 , HOXB3/HOXB4 , HOXA3 , HOXA5 , Chr1(q21.1).A, and Chr6(p22.1). In particular the OSR1 , SIM1 and HOXB3/HOXB4 regions demonstrated high potential as biomarkers in LAC. For OSR1 , hypermethylation was detected in 47/48 LAC cases compared to 1/31 tumor-adjacent normal lung samples. Similarly, 45/49 and 36/48 LAC cases compared to 3/31 and 0/31 tumor-adjacent normal lung samples showed hypermethylation of the SIM1 and HOXB3/HOXB4 regions, respectively. In conclusion, this study has identified and validated 15 DMRs that can be targeted as biomarkers in LAC.
Type of Medium:
Online Resource
ISSN:
2045-2322
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2016
detail.hit.zdb_id:
2615211-3
Bookmarklink