In:
British Journal of Nutrition, Cambridge University Press (CUP), Vol. 123, No. 7 ( 2020-04-14), p. 737-755
Abstract:
The present study investigated the effects of condensed tannins (CT) on intestinal immune function in on-growing grass carp ( Ctenopharyngodon idella ). A total of 540 healthy grass carp were fed six diets containing different levels of CT (0, 10·00, 20·00, 30·00, 40·00 and 50·00 g/kg diet) for 70 d and then challenged with Aeromonas hydrophila for 14 d. The results showed that, compared with the control group, dietary CT (1) induced intestinal histopathological lesions and aggravated enteritis; (2) decreased lysozyme and acid phosphatase activities, complement 3 (C3), C4 and IgM contents and down-regulated the Hepcidin , liver-expressed antimicrobial peptide ( LEAP ) -2A , LEAP-2B , Mucin2 and β-defensin-1 mRNA levels in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI) ( P 〈 0·05); (3) down-regulated the mRNA levels of anti-inflammatory cytokines transforming growth factor ( TGF ) -β1 , TGF-β2 (not in MI and DI), IL-4/13A (not IL-4/13B ), IL-10 and IL-11 partly correlated with target of rapamycin (TOR) signalling; and (4) up-regulated the mRNA levels of pro-inflammatory cytokines interferon-γ2 , IL-1β , IL-6 , IL-8 (not in PI), IL-12p35 , IL-12p40 , IL-15 and IL-17D partly related to NF-κB signalling in the intestine of on-growing grass carp. Overall, the results indicated that CT could impair the intestinal immune function, and its potential regulation mechanisms were partly associated with the TOR and NF-κB signalling pathways. Finally, based on the percentage weight gain and enteritis morbidity, the maximum allowable levels of CT for on-growing grass carp (232·22–890·11 g) were estimated to be 18·6 and 17·4 g/kg diet, respectively.
Type of Medium:
Online Resource
ISSN:
0007-1145
,
1475-2662
DOI:
10.1017/S0007114519003295
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2020
detail.hit.zdb_id:
2016047-1
SSG:
12
SSG:
21
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