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1

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Characterization of opioid peptides in the rat stomach

Nishimura, Erica ; Kwok, Yin Nam ; McIntosh, Christopher H. S.

Canadian Science Publishing ; 1986

In: Biochemistry and Cell Biology Vol. 64, No. 8 ( 1986-08-01), p. 733-742

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In: Biochemistry and Cell Biology, Canadian Science Publishing, Vol. 64, No. 8 ( 1986-08-01), p. 733-742

Abstract: A combination of several chromatographic and assay systems was used to characterize the opioid peptides in rat stomach extracts. Partial purification of opioid material in acetic acid extracts of the corpus plus antrum regions of the rat stomach was carried out by gel filtration chromatography on Sephadex G-50, followed by adsorption onto Amberlite XAD-2 resin. A single peak in opioid activity was determined by both radioreceptor assay (RRA) and bioassay. By high performance liquid chromatography, this peak was resolved into five distinct components, characterized by RRA and (or) radioimmunoassay, with retention times corresponding to methionine enkephalin (met-enk), leucine enkephalin, met-enk-arg 6 -gly 7 -leu 8 , met-enk-arg 6 -phe 7 , and dynorphin 1–13. Closer examination of the dynorphin component revealed the presence of dynorphins 1–17, 1–13, and 1–8. Trypsin digestion of the partially purified (Sephadex G-50 and Amberlite XAD-2 chromatographed) extract resulted in an overall increase in opioid activity, suggesting the presence of larger, possibly precursor forms.

Type of Medium: Online Resource

ISSN: 0829-8211 , 1208-6002

URL: Article

DOI: 10.1139/o86-100

Language: English

Publisher: Canadian Science Publishing

Publication Date: 1986

SSG: 12

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2

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Presence and actions of vasoactive intestinal peptide in the isolated rabbit heart

Accili, Eric A. ; Buchan, Alison M. J. ; Kwok, Yin Nam ; [et al.]

Canadian Science Publishing ; 1995

In: Canadian Journal of Physiology and Pharmacology Vol. 73, No. 1 ( 1995-01-01), p. 134-139

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In: Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 73, No. 1 ( 1995-01-01), p. 134-139

Abstract: The objectives of these experiments were to localize vasoactive intestinal peptide immunoreactivity (VIP-IR) in the intracardiac ganglia of the interatrial septum of the rabbit heart and to examine the effects of vasoactive intestinal peptide (VIP) on the isolated perfused rabbit heart. Cell bodies of neurones containing VIP-IR were located in the interatrial septa of rabbit hearts by using immunocytochemistry. In addition, the effects of substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) on the isolated rabbit heart were compared with those of VIP. Bolus injections of SP, NKA, VIP, and NKB caused dose-dependent decreases in the perfusion pressure of hearts perfused at constant flow with ED 50 values of 2.73 ± 0.10 fmol (n = 6), 0.18 ± 0.01 pmol (n = 8), 93.75 ± 1.88 pmol (n = 6), and 75.00 ± 3.06 pmol (n = 6), respectively. The slope of the dose–response curve of VIP was much greater than those of SP and NKA, suggesting the presence of one receptor subtype for VIP and multiple receptor subtypes for the neurokinins on rabbit coronary vessels. Differences in the slopes of the dose–response curves and potency may reflect differences in the mechanism of vasodilatation. The maximal values of vasodilatation of all of the peptides did not differ. None of the peptides produced significant changes in heart rate, left ventricular pressure, or contractility. These results suggest that VIP found in the intracardiac neurones of the rabbit heart can mediate coronary vasodilatation.Key words: coronary vasodilatation, intracardiac ganglia, substance P, neurokinin A, neurokinin B.

Type of Medium: Online Resource

ISSN: 0008-4212 , 1205-7541

URL: Article

DOI: 10.1139/y95-019

Language: English

Publisher: Canadian Science Publishing

Publication Date: 1995

detail.hit.zdb_id: 2004356-9

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3

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Release of substance P-like immunoreactivity from the vascularly perfused rat stomach

Kwok, Yin Nam ; McIntosh, Christopher H.S.

Elsevier BV ; 1990

In: European Journal of Pharmacology Vol. 180, No. 2-3 ( 1990-5), p. 201-207

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In: European Journal of Pharmacology, Elsevier BV, Vol. 180, No. 2-3 ( 1990-5), p. 201-207

Type of Medium: Online Resource

ISSN: 0014-2999

URL: Article

DOI: 10.1016/0014-2999(90)90303-N

Language: English

Publisher: Elsevier BV

Publication Date: 1990

detail.hit.zdb_id: 1483526-5

SSG: 15,3

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4

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Stimulus-specific inhibition of insulin release from rat pancreas by both rat and porcine galanin

Bruce Verchere, C. ; Kwok, Yin Nam ; Brown, John C.

Elsevier BV ; 1992

In: Life Sciences Vol. 51, No. 25 ( 1992-1), p. 1945-1951

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In: Life Sciences, Elsevier BV, Vol. 51, No. 25 ( 1992-1), p. 1945-1951

Type of Medium: Online Resource

ISSN: 0024-3205

URL: Article

DOI: 10.1016/0024-3205(92)90111-2

Language: English

Publisher: Elsevier BV

Publication Date: 1992

detail.hit.zdb_id: 2013911-1

SSG: 12

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5

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Improved blood glucose disposal and altered insulin secretion patterns in adenosine A 1 receptor knockout mice

Yang, Gary K. ; Fredholm, Bertil B. ; Kieffer, Timothy J. ; [et al.]

American Physiological Society ; 2012

In: American Journal of Physiology-Endocrinology and Metabolism Vol. 303, No. 2 ( 2012-07-15), p. E180-E190

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In: American Journal of Physiology-Endocrinology and Metabolism, American Physiological Society, Vol. 303, No. 2 ( 2012-07-15), p. E180-E190

Abstract: Type 2 diabetes mellitus (T2DM) is characterized by the inability of the pancreatic β-cells to secrete enough insulin to meet the demands of the body. Therefore, research of potential therapeutic approaches to treat T2DM has focused on increasing insulin output from β-cells or improving systemic sensitivity to circulating insulin. In this study, we examined the role of the A 1 receptor in glucose homeostasis with the use of A 1 receptor knockout mice (A 1 R −/− ). A 1 R −/− mice exhibited superior glucose tolerance compared with wild-type controls. However, glucose-stimulated insulin release, insulin sensitivity, weight gain, and food intake were comparable between the two genotypes. Following a glucose challenge, plasma glucagon levels in wild-type controls decreased, but this was not observed in A 1 R −/− mice. In addition, pancreas perfusion with oscillatory glucose levels of 10-min intervals produced a regular pattern of pulsatile insulin release with a 10-min cycling period in wild-type controls and 5 min in A 1 R −/− mice. When the mice were fed a high-fat diet (HFD), both genotypes exhibited impaired glucose tolerance and insulin resistance. Increased insulin release was observed in HFD-fed mice in both genotypes, but increased glucagon release was observed only in HFD-fed A 1 R −/− mice. In addition, the regular patterns of insulin release following oscillatory glucose perfusion were abolished in HFD-fed mice in both genotypes. In conclusion, A 1 receptors in the pancreas are involved in regulating the temporal patterns of insulin release, which could have implications in the development of glucose intolerance seen in T2DM.

Type of Medium: Online Resource

ISSN: 0193-1849 , 1522-1555

URL: Article

DOI: 10.1152/ajpendo.00050.2012

Language: English

Publisher: American Physiological Society

Publication Date: 2012

detail.hit.zdb_id: 1477331-4

SSG: 12

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6

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Cysteamine-induced reduction in gastrointestinal somatostatin: evidence for a region-specific loss in immunoreactivity

McIntosh, Christopher H.S. ; Bakich, Victoria ; Bokenfohr, Kerry ; [et al.]

Elsevier BV ; 1988

In: Regulatory Peptides Vol. 21, No. 3-4 ( 1988-6), p. 205-218

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In: Regulatory Peptides, Elsevier BV, Vol. 21, No. 3-4 ( 1988-6), p. 205-218

Type of Medium: Online Resource

ISSN: 0167-0115

URL: Article

DOI: 10.1016/0167-0115(88)90003-1

Language: English

Publisher: Elsevier BV

Publication Date: 1988

detail.hit.zdb_id: 1498712-0

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7

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WITHDRAWN: Involvement of Adenosine Signaling in Controlling the Release of Ghrelin from the Mouse Stomach

Yang, Gary K. ; Yip, Linda ; Fredholm, Bertil B. ; [et al.]

Elsevier BV ; 2011

In: Regulatory Peptides ( 2011-1)

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In: Regulatory Peptides, Elsevier BV, ( 2011-1)

Type of Medium: Online Resource

ISSN: 0167-0115

URL: Article

DOI: 10.1016/j.regpep.2011.01.002

Language: English

Publisher: Elsevier BV

Publication Date: 2011

detail.hit.zdb_id: 1498712-0

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8

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Galanin inhibition of cholecystokinin-8-induced increase in [Ca2+]i in individual rat pancreatic B-cells

Wang, Jian ; Kwok, Yin Nam ; Baimbridge, Kenneth G. ; [et al.]

Elsevier BV ; 1992

In: Biochemical and Biophysical Research Communications Vol. 182, No. 2 ( 1992-01), p. 858-863

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In: Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 182, No. 2 ( 1992-01), p. 858-863

Type of Medium: Online Resource

ISSN: 0006-291X

URL: Article

DOI: 10.1016/0006-291X(92)91811-4

RVK:

WA 15000

Language: English

Publisher: Elsevier BV

Publication Date: 1992

detail.hit.zdb_id: 1461396-7

SSG: 12

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9

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Dihydroergocryptine binding and α-adrenoreceptors in smooth muscle

KUNOS, GEORGE ; HOFFMAN, BRIAN ; KWOK, YIN NAM ; [et al.]

Springer Science and Business Media LLC ; 1979

In: Nature Vol. 278, No. 5701 ( 1979-3), p. 254-256

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In: Nature, Springer Science and Business Media LLC, Vol. 278, No. 5701 ( 1979-3), p. 254-256

Type of Medium: Online Resource

ISSN: 0028-0836 , 1476-4687

URL: Article

DOI: 10.1038/278254a0

RVK:

TA 1000

RVK:

UA 1000

RVK:

WA 15000

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 1979

detail.hit.zdb_id: 120714-3

detail.hit.zdb_id: 1413423-8

SSG: 11

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10

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Glucose decreases extracellular adenosine levels in isolated mouse and rat pancreatic islets

Yang, Gary K. ; Squires, Paul E. ; Tian, Faming ; [et al.]

Informa UK Limited ; 2012

In: Islets Vol. 4, No. 1 ( 2012-01), p. 64-70

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In: Islets, Informa UK Limited, Vol. 4, No. 1 ( 2012-01), p. 64-70

Type of Medium: Online Resource

ISSN: 1938-2014 , 1938-2022

URL: Article

DOI: 10.4161/isl.19037

Language: English

Publisher: Informa UK Limited

Publication Date: 2012

detail.hit.zdb_id: 2589489-4

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Kooperativer Bibliotheksverbund

Berlin Brandenburg