In:
Environmental Toxicology and Chemistry, Wiley, Vol. 20, No. 8 ( 2001-08), p. 1648-1655
Abstract:
A solid‐phase microextraction (SPME) method was applied to study microcystin (MC) profiles in a natural Microcystis sp. bloom in a freshwater pond in Guangzhou, China. Three dominant MC variants, namely MC‐LR, MC‐YR, and MC‐RR, were quantified. Simultaneous study of their total, extracellular, and intracellular profiles was made possible using SPME coupled to high‐performance liquid chromatography. The total and intracellular concentrations of MC‐LR in the bloom were 8.67 × 10 −2 μg/ml and 1.93 mg/g, respectively. The corresponding concentrations of MC‐YR were 1.20 × 10 −3 μg/ml and 0.06 mg/g, respectively, and those of MC‐RR were 5.57 × 10 −2 μg/ml and 1.49 mg/g, respectively. Only MC‐LR was detectable in the extracellular phase (1.49 × 10 −2 μg/ml) of the bloom, and its concentration was 14% of the intracellular content. Mass balance consideration revealed that only 71.1% of total MC‐LR, 36.0% of total MC‐YR, and 67.4% of total MC‐RR within the cyanobacterial cells were released into the aqueous phase immediately after cell lysis.
Type of Medium:
Online Resource
ISSN:
0730-7268
,
1552-8618
DOI:
10.1002/etc.5620200805
Language:
English
Publisher:
Wiley
Publication Date:
2001
detail.hit.zdb_id:
2027441-5
SSG:
12
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