In:
European Journal of Forest Pathology, Wiley, Vol. 29, No. 3 ( 1999-06), p. 169-188
Abstract:
Oligonucleotide primers were developed for the polymerase chain reaction (PCR)‐based detection of selected Phytophthora species which are known to cause root‐rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)‐fragments presented in this paper. All three primer pairs revealed no undesirable cross‐reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula , and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg ( P. cambivora ), 4pg ( P. quercina ), and 2pg ( P. citricola ) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina , and P. cambivora in seedlings of pendunculate oak ( Quercus robur ) and European beech ( Fagus sylvatica ) which were artificially infected under controlled conditions.
Type of Medium:
Online Resource
ISSN:
0300-1237
DOI:
10.1046/j.1439-0329.1999.00141.x
Language:
English
Publisher:
Wiley
Publication Date:
1999
detail.hit.zdb_id:
2020304-4
SSG:
23
SSG:
12
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