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  • 1
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2013
    In:  Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences Vol. 67, No. 3 ( 2013-10-1), p. 224-228
    In: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, Walter de Gruyter GmbH, Vol. 67, No. 3 ( 2013-10-1), p. 224-228
    Abstract: Accessions with no germinating seeds are a common problem in plant gene banks and research institutions. Our goal was to elaborate and apply an in vitro method of germination and multiplication of old aged seeds of red and alsike clover and alfalfa. Eighteen clover and five alfalfa accessions were used for germination in vitro. Most of the accessions had produced seeds more than 20 years ago and the seeds did not germinate in soil. Seed pre-treatment with different concentrations of potassium permanganate, as well as addition of phytohormones, AgNO3 and activated carbon to germinating media were tested. Plantlets for all germinated accessions were obtained, even in the case when seeds were 44-year-old (alfalfa). Germination rate in vitro not always correlated with seed age and ranged from 2 to 72%. Pre-treatment with potassium permanganate was effective both for seeds sterilisation and germination stimulation. Most germinated seeds formed phenotypically normal seedlings with all organs. In vitro multiplication of obtained clover plants was performed. Best results were achieved by micropropagation of stem segments approximately 2 mm in length. Number of finally obtained plants depended not only on seed germination ability, but also on micropropagation ability in relation to genotype, and on acclimatization success in soil after in vitro cultivation
    Type of Medium: Online Resource
    ISSN: 1407-009X
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2013
    detail.hit.zdb_id: 2487550-8
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  • 2
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2018
    In:  Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. Vol. 72, No. 6 ( 2018-12-01), p. 341-348
    In: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., Walter de Gruyter GmbH, Vol. 72, No. 6 ( 2018-12-01), p. 341-348
    Abstract: According to the Global Strategy for Plant Conservation, 75% of endangered species should be preserved in ex situ collections till 2020. The genus Liparis has a lot of recognised taxons, but only one species, Liparis loeselii (L.) Rich., grows in Europe. L. loeselii is a rare and endangered orchid species occurring in Europe. In Latvia L. loeselii is classified as the third category of endangered and protected species. To develop the best conservation strategy, the knowledge concerning the genetic differences of protected plants in a particular area is crucial. For this purpose, the genetic diversity of L. loeselii populations from different Latvian habitats was tested. The inter-retrotransposon amplified polymorphism method (iPBS) was used for population genetic diversity evolution. In total, 54 accessions from nine habitats were collected and analysed. L. loeselii leaves have a high content of phenols that reduce the quality of extracted DNA. It was found that the percentage of polymorph loci varied among the populations of L. loeselii growing in different habitats; some of the populations were genetically homogeneous. The genetic diversity levels of L. loeselii populations are related with the population age and the growing conditions.
    Type of Medium: Online Resource
    ISSN: 1407-009X
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2018
    detail.hit.zdb_id: 2487550-8
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  • 3
    Online Resource
    Online Resource
    Rezekne Academy of Technologies ; 2015
    In:  Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference Vol. 1 ( 2015-08-03), p. 166-
    In: Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference, Rezekne Academy of Technologies, Vol. 1 ( 2015-08-03), p. 166-
    Abstract: We have carried out restoring of some Latvian alfalfa genetic resources from seeds of accessions that did not germinate in soil at all. Two of them were repatriated from the N. Vavilov All-Russian Institute of Plant Industry (multiplied more than 40 years ago) and three from the Research Institute of Agriculture (Latvia) (multiplied 15-20 years ago). For germination of old seeds we used early elaborated in vitro culture conditions. Germination rate ranged 2-60%, depending from the genotype and seeds storage conditions. Plantlets with well developed roots and 2-3 leaves were planted in the substrate in small pots and grown in a greenhouse about a month, then replanted in the soil in field conditions and grown till the maturity. Restored accessions are available now for evaluation and involving in the alfalfa breeding programs.
    Type of Medium: Online Resource
    ISSN: 2256-070X , 1691-5402
    Language: Unknown
    Publisher: Rezekne Academy of Technologies
    Publication Date: 2015
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  • 4
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2014
    In:  Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. Vol. 68, No. 3-4 ( 2014-8-1), p. 142-147
    In: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., Walter de Gruyter GmbH, Vol. 68, No. 3-4 ( 2014-8-1), p. 142-147
    Abstract: Darba mçríis bija uzlabot dubultoto haploîdu (DH) lîniju iegûðanas metodiku ar putekðnîcu kultûru palîdzîbu Latvijâ svarîgiem graudaugiem: mieþiem, vasaras un ziemas kvieðiem. Kâ izejas materiâls pçtîjumam kalpoja F1 un F2 paaudzes selekcionâru iegûtie hibrîdi. Pçtîjuma rezultâti parâdîja, ka vara sulfâta pievienoðana koncentrâcijâ 2,5 mg/l mieþu pirmapstrâdes barotnei un gan mieþu (C3), gan kvieðu (ðíidrâ AMC) kallusu indukcijas barotnçm paaugstina zaïo augu-reìenerantu iznâkumu. Liela skaita DH lîniju iegûðanu ar putekðnîcu kultûras palîdzîbu var organizçt divos etapos: pirmajâ nosaka hibrîdu atsaucîbu putekðnîcu kultûrâ un otrajâ masveidâ izmanto tikai atlasîtos hibrîdus ar augstu spçju veidot zaïus augus-reìenerantus
    Type of Medium: Online Resource
    ISSN: 1407-009X
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2014
    detail.hit.zdb_id: 2487550-8
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  • 5
    Online Resource
    Online Resource
    Rezekne Academy of Technologies ; 2007
    In:  Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference Vol. 1 ( 2007-06-23), p. 262-
    In: Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference, Rezekne Academy of Technologies, Vol. 1 ( 2007-06-23), p. 262-
    Abstract: ‘Camelina sativa’ is one of the oldest oil crops in Europe. Growing areas were reduced dramatically in 20-40-ties of the last century. Low price of rape oil and unclear composition of camelina oil were primary reasons of this process. Nevertheless, last years ‘Camelina sativa' attracted a great interest of scientists and oil processors as a crop for diversification of agriculture and, in the same time, for producing of the high quantity and quality oil for biofuel, feeding, food, and pharmacy (source of a-linolenic acid, linoleic acid and vitamins, especially E). In Latvia ‘Camelina sativa' is spread as a wild form but for the agriculture it is a new crop.The potential of me of ‘Camelina sativa’ in the Latgale region as an alternative oil crop and a new plant in the crop rotation was investigated. We compared the oil content and it composition in ‘Camelina sativa ’ seeds from plants of variety ‘Ligena’ grown in different conditions (Latvia and Germany). We also are looking for a possibility to use biotechnology methods for obtaining additional camelina breeding source material. For this purpose we applied calli culture method for producing plants-regenerants. 
    Type of Medium: Online Resource
    ISSN: 2256-070X , 1691-5402
    Language: Unknown
    Publisher: Rezekne Academy of Technologies
    Publication Date: 2007
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  • 6
    Online Resource
    Online Resource
    Rezekne Academy of Technologies ; 2015
    In:  Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference Vol. 1 ( 2015-08-03), p. 160-
    In: Environment. Technology. Resources. Proceedings of the International Scientific and Practical Conference, Rezekne Academy of Technologies, Vol. 1 ( 2015-08-03), p. 160-
    Abstract: Flax (Linum usitatissimum L.) is one of the oldest fibre and oil crop in Europe, including Latvia. In the middle of the last century growing areas of this crop was reduced dramatically. Nowadays flax attracted a great interest again as a fibre and oil processor, as well as a crop for diversification of agriculture and, in the same time, as a crop for producing high quality fibre for industry, high quantity oil for biofuel and high quality oil for feeding, food, and pharmacy (source of α-linolenic acid, linoleic acid and vitamins). We evaluated all available flax accessions of the Latvian origin and number of their hybrid lines. Agronomically important qualitative and quantitative traits, such as total plant height, technical plant height, number of seed vessels, logging resistance, vegetation period, yield of straws and seeds, 1000 seeds weight, bast fibre, number of seeds in a seed vessel, oil content and rust resistance gene L alleles L2 and L9 were recorded. Results of the field trials of two last years were summarized. Local accessions are useful as a source for breeding to adaptation of flax varieties to the local Latvian conditions. With use of molecular methods flax rust resistance allele L2 and L9 were found in eleven accessions.
    Type of Medium: Online Resource
    ISSN: 2256-070X , 1691-5402
    Language: Unknown
    Publisher: Rezekne Academy of Technologies
    Publication Date: 2015
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