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Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius

Thompson, Ann H. ; Studholme, David J. ; Green, Edward M. ; [et al.]

Springer Science and Business Media LLC ; 2008

In: Biotechnology Letters Vol. 30, No. 8 ( 2008-8), p. 1359-1365

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In: Biotechnology Letters, Springer Science and Business Media LLC, Vol. 30, No. 8 ( 2008-8), p. 1359-1365

Type of Medium: Online Resource

ISSN: 0141-5492 , 1573-6776

URL: Article

DOI: 10.1007/s10529-008-9698-1

Language: English

Publisher: Springer Science and Business Media LLC

Publication Date: 2008

detail.hit.zdb_id: 2012203-2

SSG: 12

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Phylogenetic analysis of transformable strains of thermophilic Bacillus species

Studholme, David J ; Jackson, Robin A ; Leak, David J

Oxford University Press (OUP) ; 1999

In: FEMS Microbiology Letters Vol. 172, No. 1 ( 1999-03), p. 85-90

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In: FEMS Microbiology Letters, Oxford University Press (OUP), Vol. 172, No. 1 ( 1999-03), p. 85-90

Type of Medium: Online Resource

ISSN: 0378-1097 , 1574-6968

URL: Issue

URL: Article

DOI: 10.1111/fml.1999.172.issue-1

DOI: 10.1111/j.1574-6968.1999.tb13454.x

RVK:

WA 15000

Language: English

Publisher: Oxford University Press (OUP)

Publication Date: 1999

detail.hit.zdb_id: 1501716-3

SSG: 12

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An integrated biorefinery concept for conversion of sugar beet pulp into value-added chemicals and pharmaceutical intermediates

Cárdenas-Fernández, Max ; Bawn, Maria ; Hamley-Bennett, Charlotte ; [et al.]

Royal Society of Chemistry (RSC) ; 2017

In: Faraday Discussions Vol. 202 ( 2017), p. 415-431

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In: Faraday Discussions, Royal Society of Chemistry (RSC), Vol. 202 ( 2017), p. 415-431

Type of Medium: Online Resource

ISSN: 1359-6640 , 1364-5498

URL: Article

DOI: 10.1039/C7FD00094D

Language: English

Publisher: Royal Society of Chemistry (RSC)

Publication Date: 2017

detail.hit.zdb_id: 1472891-6

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Centrifugal partition chromatography in a biorefinery context: Optimisation and scale-up of monosaccharide fractionation from hydrolysed sugar beet pulp

Ward, David P. ; Hewitson, Peter ; Cárdenas-Fernández, Max ; [et al.]

Elsevier BV ; 2017

In: Journal of Chromatography A Vol. 1497 ( 2017-05), p. 56-63

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In: Journal of Chromatography A, Elsevier BV, Vol. 1497 ( 2017-05), p. 56-63

Type of Medium: Online Resource

ISSN: 0021-9673

URL: Article

DOI: 10.1016/j.chroma.2017.03.003

RVK:

VA 5050.A

RVK:

VG 7204

RVK:

IE 1100

RVK:

VA 1120

Language: English

Publisher: Elsevier BV

Publication Date: 2017

detail.hit.zdb_id: 1491247-8

detail.hit.zdb_id: 1171488-8

SSG: 11

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Genome-Wide Association and Trans-ethnic Meta-Analysis for Advanced Diabetic Kidney Disease: Family Investigation of Nephropathy and Diabetes (FIND)

Iyengar, Sudha K. ; Sedor, John R. ; Freedman, Barry I. ; [et al.]

Public Library of Science (PLoS) ; 2015

In: PLOS Genetics Vol. 11, No. 8 ( 2015-8-25), p. e1005352-

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In: PLOS Genetics, Public Library of Science (PLoS), Vol. 11, No. 8 ( 2015-8-25), p. e1005352-

Type of Medium: Online Resource

ISSN: 1553-7404

URL: Article

DOI: 10.1371/journal.pgen.1005352

DOI: 10.1371/journal.pgen.1005352.g001

DOI: 10.1371/journal.pgen.1005352.g002

DOI: 10.1371/journal.pgen.1005352.t001

DOI: 10.1371/journal.pgen.1005352.t002

DOI: 10.1371/journal.pgen.1005352.t003

DOI: 10.1371/journal.pgen.1005352.s001

DOI: 10.1371/journal.pgen.1005352.s002

DOI: 10.1371/journal.pgen.1005352.s003

DOI: 10.1371/journal.pgen.1005352.s004

DOI: 10.1371/journal.pgen.1005352.s005

DOI: 10.1371/journal.pgen.1005352.s006

DOI: 10.1371/journal.pgen.1005352.s007

DOI: 10.1371/journal.pgen.1005352.s008

DOI: 10.1371/journal.pgen.1005352.s009

DOI: 10.1371/journal.pgen.1005352.s010

DOI: 10.1371/journal.pgen.1005352.s011

DOI: 10.1371/journal.pgen.1005352.s012

DOI: 10.1371/journal.pgen.1005352.s013

DOI: 10.1371/journal.pgen.1005352.s014

DOI: 10.1371/journal.pgen.1005352.s015

Language: English

Publisher: Public Library of Science (PLoS)

Publication Date: 2015

detail.hit.zdb_id: 2186725-2

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Characterisation of a catabolic epoxide hydrolase from a Corynebacterium sp.

Misawa, Elisa ; Chan Kwo Chion, Chan K. C. ; Archer, Ian V. ; [et al.]

Wiley ; 1998

In: European Journal of Biochemistry Vol. 253, No. 1 ( 1998-04), p. 173-183

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In: European Journal of Biochemistry, Wiley, Vol. 253, No. 1 ( 1998-04), p. 173-183

Type of Medium: Online Resource

ISSN: 0014-2956 , 1432-1033

URL: Article

DOI: 10.1046/j.1432-1327.1998.2530173.x

RVK:

WA 15000

Language: English

Publisher: Wiley

Publication Date: 1998

detail.hit.zdb_id: 1398347-7

detail.hit.zdb_id: 2172518-4

SSG: 12

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Genome-scale metabolic modeling of P. thermoglucosidasius NCIMB 11955 reveals metabolic bottlenecks in anaerobic metabolism

Mol, Viviënne ; Bennett, Martyn ; Sánchez, Benjamín J. ; [et al.]

Elsevier BV ; 2021

In: Metabolic Engineering Vol. 65 ( 2021-05), p. 123-134

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In: Metabolic Engineering, Elsevier BV, Vol. 65 ( 2021-05), p. 123-134

Type of Medium: Online Resource

ISSN: 1096-7176

URL: Article

DOI: 10.1016/j.ymben.2021.03.002

Language: English

Publisher: Elsevier BV

Publication Date: 2021

detail.hit.zdb_id: 1471017-1

SSG: 12

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Comparison of Nile Red and Cell Size Analysis for High‐Throughput Lipid Estimation Within Oleaginous Yeast

Hicks, Robert H. ; Chuck, Christopher J. ; Scott, Roderick J. ; [et al.]

Wiley ; 2019

In: European Journal of Lipid Science and Technology Vol. 121, No. 11 ( 2019-11)

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In: European Journal of Lipid Science and Technology, Wiley, Vol. 121, No. 11 ( 2019-11)

Abstract: With growing interest in oleaginous yeast as producers of future fuels and bulk chemicals, a robust, high‐throughput method for estimating lipid production is required. Although the lipophilic dye Nile red is frequently used to assay large samples of yeast and microalgae, inconsistent stain permeability between species and strains limits its effectiveness for some microorganisms. In this study, the oleaginous yeast Metschnikowia pulcherrima is used to develop a fluorescence‐free, cell‐size‐based image analysis method for estimating lipid production, which is then compared with an optimized Nile red method across several experimental scenarios. Cell size analysis (CSA) outperforms Nile red in all scenarios, correlating well with lipid extraction data when screening multiple strains, screening a subset of strains grown in different conditions, and tracking the lipid accumulation of a culture over time. Stain permeability is shown to vary significantly among the strains trialled, with lipid droplet size and cell wall thickness having a deleterious effect in the permeability of high‐lipid‐accumulating cells. CSA can also allow culture population dynamics to be monitored, providing key process information of cell size distribution in response to changing media compositions. Practical Applications : Nile red is currently the go‐to method for high‐ throughput lipid screening; however, staining inconsistencies in some organisms caused by varying cell morphology makes it challenging to optimize a robust protocol. Although fluorescence‐free methods exist (Raman spectroscopy, Fourier‐transform infrared spectroscopy, GCMS), the need for extensive sample preparation and specialist equipment restricts their widespread adoption. The CSA method presented here offers an accurate, robust, and cheap alternative for the study of microorganisms where fluorescence‐based avenues are not feasible. Furthermore, the population dynamics collected during CSA can easily be applied to bioreactor style processing, where tracking size distributions can provide real time information of culture status. This additional information is valuable even if fluorescence screening is a possibility.

Type of Medium: Online Resource

ISSN: 1438-7697 , 1438-9312

URL: Issue

URL: Article

DOI: 10.1002/ejlt.v121.11

DOI: 10.1002/ejlt.201800355

Language: English

Publisher: Wiley

Publication Date: 2019

detail.hit.zdb_id: 2012720-0

SSG: 21

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Chemoenzymic resolution and deracemisation of (±)-1-methyl-1,2-epoxycyclohexane: the synthesis of (1-S, 2-S)-1-methylcyclohexane-1,2-diol

Archer, Ian V.J. ; Leak, David J. ; Widdowson, David A.

Elsevier BV ; 1996

In: Tetrahedron Letters Vol. 37, No. 48 ( 1996-11), p. 8819-8822

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In: Tetrahedron Letters, Elsevier BV, Vol. 37, No. 48 ( 1996-11), p. 8819-8822

Type of Medium: Online Resource

ISSN: 0040-4039

URL: Article

DOI: 10.1016/S0040-4039(96)01998-3

RVK:

VA 7560

Language: English

Publisher: Elsevier BV

Publication Date: 1996

detail.hit.zdb_id: 2007074-3

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Ionic liquid pretreatment of lignocellulosic biomass with ionic liquid–water mixtures

Brandt, Agnieszka ; Ray, Michael J. ; To, Trang Q. ; [et al.]

Royal Society of Chemistry (RSC) ; 2011

In: Green Chemistry Vol. 13, No. 9 ( 2011), p. 2489-

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In: Green Chemistry, Royal Society of Chemistry (RSC), Vol. 13, No. 9 ( 2011), p. 2489-

Type of Medium: Online Resource

ISSN: 1463-9262 , 1463-9270

URL: Article

DOI: 10.1039/c1gc15374a

Language: English

Publisher: Royal Society of Chemistry (RSC)

Publication Date: 2011

detail.hit.zdb_id: 1485110-6

detail.hit.zdb_id: 2006274-6

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