In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 47, No. 12 ( 2003-12), p. 3719-3723
Abstract:
The
resistance to ampicillin and nalidixic acid in Shigella sonnei isolates obtained in Korea during the period 1998 to 2000 was
characterized. Recently (J. Y. Oh, H. S. Yu, S. K. Kim, S. Y. Seol, D. T. Cho, and
J. C. Lee, J. Clin. Microbiol. 41: 421-423,
2003) ampicillin and nalidixic acid resistance was found in 49 and 70%, respectively, of the 67 S. sonnei isolates
obtained during this period. We analyzed 138 S. sonnei isolates collected during the same period. Ampicillin and nalidixic
acid resistance was found in 30 and 86% of the isolates, respectively. The ampicillin resistance was mediated by a TEM-1β
-lactamase, and TEM-52 extended-spectrum β-lactamase was identified in one sporadic S. sonnei isolate from 1999. bla TEM-1 and bla TEM-52 were
located in conjugative R-plasmids. Tn 3 was detected in
41% of the ampicillin-resistant isolates. The R-plasmids from the transconjugants that transferred resistance to ampicillin exhibited
different restriction fragment length polymorphism patterns, and a bla TEM-1 probe was hybridized with the different
fragments. The nalidixic acid resistance was exclusively associated with an amino acid substitution, Ser83→Leu (TCG→TTG),
in gyrA . These findings indicate that the genetically related S. sonnei strains readily acquire resistance to ampicillin,
streptomycin, trimethoprim, and sulfamethoxazole but not nalidixic acid through conjugative R-plasmids from difference sources when confronted
by antibiotic selective pressures.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.47.12.3719-3723.2003
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2003
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
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