In:
American Journal of Physiology-Cell Physiology, American Physiological Society, Vol. 275, No. 2 ( 1998-08-01), p. C484-C495
Abstract:
Monolayers of the human colonic epithelial cell line T84 exhibit electrogenic Cl − secretion in response to the Ca 2+ agonist thapsigargin and to the cAMP agonist forskolin. To evaluate directly the regulation of apical Cl − conductance by these two agonists, we have utilized amphotericin B to permeabilize selectively the basolateral membranes of T84 cell monolayers. We find that apical anion conductance is stimulated by both forskolin and thapsigargin but that these conductances are differentially sensitive to the anion channel blocker DIDS. DIDS inhibits thapsigargin-stimulated responses completely but forskolin responses only partially. Furthermore, the apical membrane anion conductances elicited by these two agonists differ in anion selectivity (for thapsigargin, I − 〉 Cl − ; for forskolin, Cl − 〉 I − ). However, the DIDS-sensitive component of the forskolin-induced conductance response exhibits anion selectivity similar to that induced by thapsigargin (I − 〉 Cl − ). Thus forskolin-induced apical anion conductance comprises at least two components, one of which has features in common with that elicited by thapsigargin.
Type of Medium:
Online Resource
ISSN:
0363-6143
,
1522-1563
DOI:
10.1152/ajpcell.1998.275.2.C484
Language:
English
Publisher:
American Physiological Society
Publication Date:
1998
detail.hit.zdb_id:
1477334-X
SSG:
12
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